Parasexual Hybridization

  • Sant Saran Bhojwani
  • Prem Kumar Dantu


Protoplasts are single cells that have been stripped of their wall exposing the plasmalemma. A special property of the protoplasts is that when brought into close contact they tend to fuse with each other irrespective of the sources of the protoplasts. The technique of fusion of isolated protoplasts from somatic cells and regeneration of hybrid plants from the fusion products, called somatic hybridization or parasexual hybridization completely bypasses the sex and, thus, allows combining genomes of two desirable parents, irrespective of their taxonomic relationship. The technique of cell fusion has been applied to produce a large number of intergeneric, intertribal or interfamily symmetric allotetraploid somatic hybrids, asymmetric hybrids and somatic hybrids. A unique advantage of parasexual hybridization is in creating new combinations of nuclear and/or cytoplasmic genomes, leading to enrichment of the current gene pool. According to Liu et al (2005) 90 intergeneric somatic hybrids have been obtained. This chapter describes the techniques of enzymatic isolation of protoplasts in large quantities, their fusion chemically and electrically, regeneration of somatic hybrids from the fused somatic cells, and application of parasexual hybridization.


Cytoplasmic Male Sterility Somatic Hybrid Hybrid Cell Protoplast Fusion Fusion Product 
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Copyright information

© Springer India 2013

Authors and Affiliations

  1. 1.Department of BotanyDayalbagh Educational Institute (Deemed University)AgraIndia

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