Curtain Raiser to Novel MAS Platforms
Plant breeding programme’s key goal revolves in generation of elite crop plants that are having combination of superior genes/alleles. However, the critical limitation is lack of understanding of what most genes do in terms of the desired phenotype expression (e.g. pest resistance, salt tolerance and yield increase) in plants. We do know that all the agronomically important traits are quite complex. For example, in halophytes, we know that salt tolerance depends on the ability to compartmentalise ions, which in turn depends on regulation of transpiration, the tight control of leakage of ions through the root apoplast, the nature of the membranes in the leaf vacuoles, synthesis of compatible solutes such as glycine betaine and the ability to tolerate low K and Na ratios in the cytoplasm of mature cells or the ability of protein synthesis to operate at low K:Na ratios in the cells, etc. Under such conditions, how QTL mapping might be useful in increasing the yield under those unfavourable environments? In order to have efficient knowledge-based MAS, it is necessary to understand the techniques that are being used to unravel the function of genes, and such knowledge should be incorporated to the QTL mapping procedure. This chapter provides the state-of-the-art techniques in molecular, biochemical and physiological studies and their potential role in MAS.
KeywordsAmplify Fragment Length Polymorphism Metabolic Network Physiological Trait Test Hybrid Representational Difference Analysis
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