Enzymatic Polymerisation

  • Soundar Divakar


Potentiality of lipases to effect esterification has been explored to prepare polymers of monomer molecules which possess hydroxyl and carboxyl functions like lactic acid, ε-caprolactone, p-hydroxybenzoic acid, p-aminobenzoic acid, adipic acid and 1,6-hexanediol. Ring opening polymerisation of ε-caprolactone gave a polycaprolactone polymer of molecular weight 10,000, which was the best compared to the low molecular weight polymers of molecular weight 400–5,000 obtained with the other above-mentioned monomers. Relative advantages and disadvantages of the use of lipases in such polymerisation reactions along with the film-forming properties of polylactic acid and polycaprolactone and its blends are presented in this chapter.


Polylactic Acid Glycolic Acid Adipic Acid Malonic Acid Succinic Anhydride 
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  1. Bartz T, Roehe P (2003) Ger Offen DE 19854404. A1, 31 May 2000, p 6Google Scholar
  2. Binns F, Roberts SM, Taylor A, Williams CF (1994) Studies leading to the large scale synthesis of polyesters using enzymes. J Chem Soc Perkin Trans 1:899–904Google Scholar
  3. Bisht KS, Deng F, Gross RA, Kaplan DL, Swift G (1998) Ethyl glucoside as a multifunctional initiator for enzyme-catalyzed regio-selective lactone ring-opening polymerization. J Am Chem Soc 120:1363–1367CrossRefGoogle Scholar
  4. Bohl W, Partusch G, Fleishmann S (2000) Ger Offen DE 19928608 A1, 28 DecGoogle Scholar
  5. Chaudhary AN, Qadri RB (1990) Higher fatty acid esters of lactic acid. Pak J Ind Res 33:334–336Google Scholar
  6. Divakar S (2003) Porcine pancreas lipase catalysed preparation of oligomers of p-hydroxybenzoic acid and p-aminobenzoic acid. Ind J Chem Sect B 42B:1467–1470Google Scholar
  7. Divakar S (2004) Porcine pancreas lipase catalysed ring-opening polymerization of ε-caprolactone. J Macromol Sci Part A Pure Appl Chem A41(5):537–546CrossRefGoogle Scholar
  8. Divakar S, Kiran KR, Harikrishna S and Karanth NG (1999) An improved process for the preparation of esters of organic acids and alcohols. Indian Patent, 1243/DEL/99 No. 191078Google Scholar
  9. Gowariker VR, Viswanathan NV, Sreedhar J (1992) Polymer science. Wiley Eastern Ltd., New DelhiGoogle Scholar
  10. Gross RA, Kumar A, Kalra B (2001) Polymer synthesis by in vitro enzyme catalysis. Chem Rev 101(7):2097–2124PubMedCrossRefGoogle Scholar
  11. Huang K, Lin YG, Winter HH (1992) p-Hydroxy benzoate/ethylene terephthalate copolyester: structure of high-melting crystals formed during partially molten state annealing. Polymer 33:4533–4537CrossRefGoogle Scholar
  12. Inoue A (1996) JPN Kokai Tokkyo Koho Jp 08059847, A25, Mar 1996, Heirei 5, Japan (CA)Google Scholar
  13. Kiran KR, Divakar S (2003) Lipase catalyzed polymerization of lactic acid and its film forming properties. World J Microbiol Biotechnol 19:859–865CrossRefGoogle Scholar
  14. Knani D, Gutman AL, Kohn DH (1993) Enzymatic polyesterification in organic media enzyme – catalyzed synthesis of linear polyesters. 1. Condensation polymerization of linear hydroxyesters. J Polym Sci Part A Polym Chem 31:1221–1232CrossRefGoogle Scholar
  15. Kobayashi S, Uyama H, Namekawa S, Hayakawa H (1998) Enzymatic ring-opening polymerization and copolymerization of 8-octanolide by lipase catalyst. Macromolecules 31:5655–5659CrossRefGoogle Scholar
  16. Kumar A, Gross RA (2000) Candida antarctica lipase B-catalyzed transesterification: new synthetic routes to copolyesters. J Am Chem Soc 122:11767–11770CrossRefGoogle Scholar
  17. Kumar A, Kumar N, Parmar V (1996) Preparative and mechanistic aspects of interesterification reactions on diols and peracetylated polyphenolic compounds catalysed by lipases. Pure Appl Chem 68:749–752CrossRefGoogle Scholar
  18. Lipinsky ES, Sinclair RG (1986) Is lactic acid a commodity chemical? Chem Eng Progr 82:26–32Google Scholar
  19. Muhlfeld H, Wagener S (2000) Eur Pat Appl EP 1043349, A2, 11 Oct 2000, 5 ppGoogle Scholar
  20. Namekawa S, Uyama H, Kobayashi S (1996) Lipase-catalyzed ring-opening polymerization and copolymerization of β-propiolactone. Polym J 28:730–731CrossRefGoogle Scholar
  21. Preston J, Kotek R, Krigbaum WR (1992) Macromol Synth 11:27Google Scholar
  22. Rika M, Tadaki S, Kayoko Y (1996) Jpn Kokkai Tokkyo Koho JP 08:73,573Google Scholar
  23. Sandez-Adsuar MS, Martin-Martiz JM (2000) J Adhes Sci Technol 14:8Google Scholar
  24. Seiji O, Masahiro O (1992) Jpn Kokai Tokkyo Koho JP 06:172502Google Scholar
  25. Seymour RB, Carrea CE Jr (1984) Structure relationship in polymers. Plannum Press, New York, pp 55–69CrossRefGoogle Scholar
  26. Svirkin YY, Xu J, Gross RA, Kaplan DL, Swift G (1996) Enzyme catalyzed stereo-selective ring- opening polymerization of α-methyl-β-propiolactone. Macromolecules 29:4591–4597CrossRefGoogle Scholar
  27. Taesler C, Wittich H, Jourgen C, Schulte K, Kricheldorf HR (1996) J Appl Polym Sci 61:783–792CrossRefGoogle Scholar
  28. Uyama H, Kobayashi S (1996) Enzymatic ring-opening polymerization of macrolides to polyesters. Front Biomed Biotechnol 3:5–15Google Scholar
  29. Voss W, Spielan P (1958) German (East) Patent 14, 548Google Scholar
  30. Zhang H, Deng X, Huang Z (1996) Ring-opening polymerization of ε-caprolactone by bacterial protease. Biotechnol Lett 18:1051–1054CrossRefGoogle Scholar

Copyright information

© Springer India 2013

Authors and Affiliations

  1. 1.Central Food Technological Research InstituteMysoreIndia

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