Abstract
Insect-resistant proteins have been one of the major successes of applying plant genetic engineering technology to agriculture. Relatively little is known about insect defense mechanisms in the lower plants. The current paradigm is that secondary metabolites and physical barriers are most important in conferring insect resistance in plants. We investigated whether protein-based resistance exists in representatives of bryophytes. We screened a total of 20 bryophytes such as Porella acutifolia, Fissidens asperifolius, Fissidens crispulus, Hypopterygium tamarisci, Brachymenium nepalense, Brachythecium buchananii, Campylopus pilifer, Marchantia linearis, Leucobryum bowringii, Plagiochila beddomei, Isopterygium albescens, Taxiphyllum taxirameum, Octoblepharum albidum, Bryum argenteum, Riccia frostii, Philonotis fontana, Racopilum cuspidigerum, Funaria hygrometrica, Pallavicinia lyellii, and Polytrichum commune for protein-based insecticidal activity against the two common lepidopteran pests: corn earworm (Helicoverpa zea) and armyworm (Spodoptera litura). Protein extracts from bryophyte species were compared with those from a lepidopteran-susceptible Glycine max cultivar (Cobb) in bioassays for insect resistance. The Octoblepharum albidum, Fissidens asperifolius, Bryum argenteum, and Marchantia linearis protein extracts caused the greatest decrease in damage in leaf-disk assays and insect larval growth. The results from dietary utilization experiments showed a reduction in efficiency of conversion of ingested food and digested food and an increase in approximate digestibility and metabolic cost. The in vivo effects of proteins of bryophytes showed varied response toward the parameters such as larval weight, growth rate, and proportion of survivors. These bryophyte species are potential candidates for further evaluation.
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Asakawa Y. Recent advances in phytochemistry of bryophytes – acetogenins, terpenoids and bis(bibenzyl)s from selected Japanese, Taiwanese, New Zealand, Argentinean and European liverworts. Phytochemistry. 2001;56:297–312.
Nishiyama T, Fugita T, Shin IT, Seki M, Nishide H, Uchiyama I, Kamiya A. Comparative genomics of Physcomitrella patens gametophytic transcriptome and Arabidopsis thaliana: implications for land plant evolution. Proc Natl Acad Sci USA. 2003;100:8007–12.
Cooper-Driver GA. Insect-fern associations. Entomol Exp Appl. 1978;24:110–6.
Davidson AJ, Harborne JB, Longton RE. Identification of hydroxycinnamic and phenolic acids in Mnium hornum and Brachythecium rutabulum and their possible role in protection against herbivory. J Hattori Bot Lab. 1989;67:415–22.
Weintraub J. Lithinine moths on ferns: a phylogenetic study of insect-plant interactions. Biol J Linn Soc. 1995;55:239–50.
Auerbach MJ, Hendrix SD. Insect-fern interactions: macro-lepidopteran utilization and species area association. Ecol Entomol. 1980;5:99–104.
Hendrix SD, Marquis RJ. Herbivore damage to 3 tropical ferns. Biotropica. 1983;15:108–11.
Asakawa Y. Biologically active substances from bryophytes. In: Chopra RN, Bhatla SC, editors. Bryophyte development: physiology and biochemistry. Boston: CRC Press; 1990. p. 259–88.
Basra A, Basra R. Mechanisms of environmental stress resistance in plants. Annu Rev Biochem. 1997;59:873–907.
Harborne JB. Introduction to ecological biochemistry. San Diego: Academic; 1993.
Cooper-Driver GA. Anti-predation strategies in pteridophytes: a biochemical approach. Proc R Soc Edinb B (Biol Sci). 1985;86B:397–402.
Lafont R, Horn DHS. Phytoecdysteroids: structure and occurrence. In: Koolman J, editor. Ecdysone: from chemistry to mode of action. Stuttgart: Thieme; 1989. p. 39–64.
Fenwick GR. Bracken Pteridium aquilinum: toxic effects and toxic constituents. J Sci Food Agric. 1988;46:147–73.
Hendrix SD. The resistance of Pteridium aquilinum to insect attack by Trichoplusia ni (Hubn.). Oecologia. 1977;26:347–61.
Waldbauer GP. The consumption and utilization of food by insects. Adv Insect Physiol. 1968;5:229–88.
Farrar RR, Barbour JD, Kennedy KG. Quantifying food consumption and growth in insects. Ann Entomol Soc Am. 1989;82:593–8.
Terra WR, Ferreira C. Insect digestive enzymes: properties, compartmentalization and function. Comp Biochem Physiol B. 1994;109:1–62.
Broadway RM. Dietary proteinase inhibitors alter the complement of midgut proteinases. Arch Insect Biochem. 1996;41:107–16.
De Leo F, Bonade’-Bottino M, Ceci R, Gallerani R, Joaunin L. Effects of a mustard trypsin inhibitor expressed in different plants on three lepidopteran pests. Insect Biochem Mol Biol. 2001;31:593–602.
McManus MT, Burgess EPJ. Effects of soybean (Kunitz) trypsin inhibitor on growth and digestive proteases of larvae Spodoptera litura. J Insect Physiol. 1995;41:731–8.
Gatehouse A, Norton MR, Davison E, Babbe GM, Newell SM, Gatehouse JA. Digestive proteolytic activity in larvae of tomato moth, Lacanobia oleracea, effects of plant proteinase inhibitor in vitro & in vivo. J Insect Physiol. 1999;45:545–58.
Morbue A, Blackwell AJ. Azadirachtin: an update. J Insect Physiol. 1993;39:903–24.
Koul O, Multani JS, Singh G, Daniewski WM, Berlozecki S. 6-β- Hydroxygedunin from Azadirachta indica. Its potentiating effects with some non-azadirachtin limonoids in neem against lepidopteran larvae. J Agric Food Chem. 2003;51:2937–42.
Wheeler DA, Isman MB. Antifeedant and toxic activity of Trichilia americana extract against the larvae of Spodoptera litura. Entomol Exp et Appl. 2001;98:9–16.
Paulillo Cesar LMS, Adriana LR, Plinio TC, Jose PRP, Walter TR, Marcio SFC. Changes in midgut endopeptidase activity of Spodoptera frugiperda (Lepidoptera: Noctuidae) are responsible for adaptation to soybean proteinase inhibitors. J Econ Entomol. 2000;93:892–6.
Cloutier Jean C, Fournier M, Yelle S, Michaud D. Adult Colorado potato beetles, Leptinotarsa decemlineata compensate for nutritional stress on Oryzacystatin 1-transgenic potato plants by hypertrophic behavior and over production of incentive proteases. Arch Insect Biochem Physiol. 2000;44:69–81.
Zhu-Salzman K, Koiwa H, Salzman RA, Shade RE, Ahn JE. Cowpea bruchid Callosobruchus maculatus uses a three-component strategy to overcome a plant defensive cysteine protease inhibitor. Insect Mol Biol. 2003;12:135–45.
Volpicella M, Ceci LR, Cordewener J, America T, Gallerani R, Bode W, Jongsma MA, Beekwilder. Properties of purified gut trypsin from Helicoverpa zea, adapted to proteinase inhibitors. Eur J Biochem. 2003;270:10–9.
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We would like to thank Kerala State Council for Science, Technology and Environment for providing financial support for the project.
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Krishnan, R., Murugan, K. (2012). Evaluation of Bryophyte Protein-Based Defense Against Selected Phytophagous Insects. In: Sabu, A., Augustine, A. (eds) Prospects in Bioscience: Addressing the Issues. Springer, India. https://doi.org/10.1007/978-81-322-0810-5_3
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DOI: https://doi.org/10.1007/978-81-322-0810-5_3
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