Isothermal Calorimetry and Raman Spectroscopy to Study Response of Plants to Abiotic and Biotic Stresses
This review presents applying a typical chemical methods such as isothermal calorimetry and Raman sectroscopy to study response of plants to aboiotic and biotic stress, so how can be used analytical techniques to better understunding of plant phisiology.
Plants produce heat during metabolism, and measurements of these metabolic heat could lead understanding of plant physiology. Calorimetric measurements have proven to be useful as monitors for many types of biological processes such as seed germination, seedlings growth or plant tissue vitality. Differences in amount of heat production give the information about how big is the impact of stress factors on plant.
Raman spectroscopy can be used for in situ analysis of valuable substances in living plant tissue. Chemical compounds (primary and secondary metabolites) content could be done as point-measurements and distribution as a Raman mapping. This techniques allow to obtain the data as a Raman-spectra, which present some characteristic bands of plants components. These bands give informations about the specific chemical composition of plant tissue. Two-dimensional Raman maps provide insight not only into the distribution, but also into relative content of the compounds in the specified area of the plant tissue.
Combination of spectroscopy and hierarchical cluster analysis enables a simple, quickly and reliable method for chemotaxonomy charakterization.
Isothermal calorimetry and Raman spectroscopy, especially combination of these two analitical techniques is a very good tool to describe the susceptibility and response of plant to abiotic and biotic stress factors.
KeywordsHeat Production Heat Emission Metabolic Heat Production Polyene Chain Isothermal Calorimetry
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