Abstract
The wide popularity of TLC is due to its sensitivity to microgram quantity, very short time of analysis, and semiquantitative information of the major active compounds, which can be documented for regulatory purposes as evidence. The major absorbent used in TLC is silica gel with fluorescent indicator in it, and a few constituents in the extract can be seen under UV light. Once the TLC plate is spotted, it is developed in a suitable mobile phase to separate the ingredients. On the completion of chromatogram, the UV non-detectable compounds can be visualized using a spray reagent and/under UV light. TLC has wide application for profession to combine the best elements of traditional healing system and modern medical practices, to improve healthcare system, as it can be used for almost every chemical class of compounds, with the exception of the highly volatile constituents.
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Joshi, D.D. (2012). TLC: Herbal Drugs and Fingerprints. In: Herbal Drugs and Fingerprints. Springer, India. https://doi.org/10.1007/978-81-322-0804-4_2
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DOI: https://doi.org/10.1007/978-81-322-0804-4_2
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