TLC: Herbal Drugs and Fingerprints
The wide popularity of TLC is due to its sensitivity to microgram quantity, very short time of analysis, and semiquantitative information of the major active compounds, which can be documented for regulatory purposes as evidence. The major absorbent used in TLC is silica gel with fluorescent indicator in it, and a few constituents in the extract can be seen under UV light. Once the TLC plate is spotted, it is developed in a suitable mobile phase to separate the ingredients. On the completion of chromatogram, the UV non-detectable compounds can be visualized using a spray reagent and/under UV light. TLC has wide application for profession to combine the best elements of traditional healing system and modern medical practices, to improve healthcare system, as it can be used for almost every chemical class of compounds, with the exception of the highly volatile constituents.
KeywordsHerbal Drug Solvent Front Santalum Album Coleus Forskohlii Turmeric Extract
- 2.Sachan AK, Sachan NK, Kumar S, Sachan A, Gangwar SS. Evaluation and standardization of essential oils for development of alternative dosage forms. Eur J Sci Res. 2010;46(2):194–203.Google Scholar
- 3.Joshi DD. Thin layer chromatography and biotechnology at molecular level. In: Textbook of molecular biotechnology. New Delhi: I.K. International Pub. House; 2009. p. 1181–96. Sample Chapter 53.Google Scholar
- 4.Klier B. Current problems with identification of herbal drugs. PhytoLab GmbH & Co. KG 91487 Vestenbergsgreuth Germany. http://www.ga-online.org/files/Graz/WS-4_Klier.pdf. Accessed 29 Mar 2012.
- 8.Alsbou E. Ambient mass spectrometry desorption electrospray ionization (DESI) & direct analysis in real time (DART). http://www.ecplaza.net/tradeleads/seller/5638482/evodiamine_98_99_hplc.html# none (2010). Accessed 28 Mar 2012.
- 9.Meena R, Meena AK, Khan SA, Mangeswari S. Evaluation of an Unani compound formulation-Majoon-e-Sandal. Int J Pharm Sci Res. 2010;1(5):238–42.Google Scholar
- Joshi DD. Utilisation of medicinal plants for health and wealth. Proceedings of national workshop on role of forestry in employment generation and rural development. IFRI Dehradun, India; 2006. p. 59–68.Google Scholar
- Kirchner JG. Thin layer chromatography. In: Techniques of chemistry. 2nd ed. New York: Wiley; 2000. XIV.Google Scholar
- Stationary Office. British Pharmacopoeia 2007. London: The Stationary Office; 2006.Google Scholar
- USP. United States pharmacopeial convention. USP 29–NF 24. Rockville: United States Pharmacopeial Convention, Inc. electronic version; 2006.Google Scholar
- Verbitski SM, et al. Rapid screening for complex mixtures by thin layer chromatography-bioluminescence. Technical article. Am Biotechnol Lab. 2006;24(9):40–1.Google Scholar
- WHO Monographs on Selected Medicinal Plants – Volume 2. http://apps.who.int/medicinedocs/en/d/Js4927e/. Accessed 28 Mar 2012.