TLC: Herbal Drugs and Fingerprints

  • Devi Datt Joshi


The wide popularity of TLC is due to its sensitivity to microgram quantity, very short time of analysis, and semiquantitative information of the major active compounds, which can be documented for regulatory purposes as evidence. The major absorbent used in TLC is silica gel with fluorescent indicator in it, and a few constituents in the extract can be seen under UV light. Once the TLC plate is spotted, it is developed in a suitable mobile phase to separate the ingredients. On the completion of chromatogram, the UV non-detectable compounds can be visualized using a spray reagent and/under UV light. TLC has wide application for profession to combine the best elements of traditional healing system and modern medical practices, to improve healthcare system, as it can be used for almost every chemical class of compounds, with the exception of the highly volatile constituents.


Herbal Drug Solvent Front Santalum Album Coleus Forskohlii Turmeric Extract 
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  1. 1.
    Abu-Hamdah S, Afifi FU, Shehadeh M, Khalid S. Simple quality control procedures for selected medicinal plants commonly used in Jordan. Pharm Biol. 2005;43(1):1–7.CrossRefGoogle Scholar
  2. 2.
    Sachan AK, Sachan NK, Kumar S, Sachan A, Gangwar SS. Evaluation and standardization of essential oils for development of alternative dosage forms. Eur J Sci Res. 2010;46(2):194–203.Google Scholar
  3. 3.
    Joshi DD. Thin layer chromatography and biotechnology at molecular level. In: Textbook of molecular biotechnology. New Delhi: I.K. International Pub. House; 2009. p. 1181–96. Sample Chapter 53.Google Scholar
  4. 4.
    Klier B. Current problems with identification of herbal drugs. PhytoLab GmbH & Co. KG 91487 Vestenbergsgreuth Germany. Accessed 29 Mar 2012.
  5. 5.
    Wohlmuth H, Penman KG, Pearson T, Lehmann RP. Pharmacognosy and chemotypes of passionflower (Passiflora incarnata L.). Biol Pharma Bull. 2010;33(6):1015–8.CrossRefGoogle Scholar
  6. 6.
    Kim HJ, Jee EH, Ahn KS, Choi HS, Jang YP. Identification of marker compounds in herbal drugs on TLC with DART-MS. Arch Pharm Res. 2010;33(9):1355–13559.PubMedCrossRefGoogle Scholar
  7. 7.
    Kim HJ, Jang YP. Direct analysis of curcumin in turmeric by DART-MS. Phytochem Anal. 2009;20(5):372–7.PubMedCrossRefGoogle Scholar
  8. 8.
    Alsbou E. Ambient mass spectrometry desorption electrospray ionization (DESI) & direct analysis in real time (DART). none (2010). Accessed 28 Mar 2012.
  9. 9.
    Meena R, Meena AK, Khan SA, Mangeswari S. Evaluation of an Unani compound formulation-Majoon-e-Sandal. Int J Pharm Sci Res. 2010;1(5):238–42.Google Scholar


  1. Di X, Kelvin KC, Hei WL, Carmen WH. Fingerprint profiling of acid hydrolyzates of polysaccharides extracted from the fruiting bodies and spores of Lingzhi by high-performance thin-layer chromatography. J Chromatogr A. 2003;1018:85–95.PubMedCrossRefGoogle Scholar
  2. Hardman R, Abu-Al-Futuh IM. The detection of isomers of 4-hydroxyisoleucine by the Jeol amino acid analyser and TLC. Planta Med. 1979;36:79–84.PubMedCrossRefGoogle Scholar
  3. Joshi DD. Utilisation of medicinal plants for health and wealth. Proceedings of national workshop on role of forestry in employment generation and rural development. IFRI Dehradun, India; 2006. p. 59–68.Google Scholar
  4. Kirchner JG. Thin layer chromatography. In: Techniques of chemistry. 2nd ed. New York: Wiley; 2000. XIV.Google Scholar
  5. Kopylova E, et al. Standardization of a complex preparation for the treatment of periodontal diseases. Pharm Chem J. 2002;36:504–6 [Translated from Khimiko-Farmatservticheskii Zhurnal. 36 (9): 44–46].CrossRefGoogle Scholar
  6. Li BY, Hu Y, Liang YZ, Xie PS, Du YP. Quality evaluation of fingerprints of herbal medicine with chromatographic data. Anal Chim Acta. 2004;514:69–77.CrossRefGoogle Scholar
  7. Pascual ME, et al. Simplified screening by TLC of plant drugs. Pharm Biol. 2002;40(2):139–43.CrossRefGoogle Scholar
  8. Sharma J, Fried B. Handbook of thin layer chromatography, Chromatographic Science Series, vol. 55. New York: Marcel Dekker; 2003.CrossRefGoogle Scholar
  9. Stationary Office. British Pharmacopoeia 2007. London: The Stationary Office; 2006.Google Scholar
  10. USP. United States pharmacopeial convention. USP 29–NF 24. Rockville: United States Pharmacopeial Convention, Inc. electronic version; 2006.Google Scholar
  11. Verbitski SM, et al. Rapid screening for complex mixtures by thin layer chromatography-bioluminescence. Technical article. Am Biotechnol Lab. 2006;24(9):40–1.Google Scholar
  12. WHO Monographs on Selected Medicinal Plants – Volume 2. Accessed 28 Mar 2012.
  13. Zhao LH, Huang CY, Shan Z, Xiang BR, Mei LH. Fingerprint analysis of psoralea corylifolia L. By HPLC and LC-MS. J Chromatogr B. 2005;821:67–74.CrossRefGoogle Scholar

Copyright information

© Springer India 2012

Authors and Affiliations

  1. 1.Amity Institute of Phytochemistry & PhytomedicineAmity University, Uttar PradeshNoidaIndia

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