Abstract
In this paper, we report the variations of amino acid residues between H5N1 and H1N1 swine flu neuraminidase sequences at protein level. Random search in NCBI Flu database resulted in Canadian viral gene and analysis using blast technique revealed sites that are variant among sequences for which 3-dimensional structures were known. PDB summary database and multiple alignments were employed for validation of the results. Based on the mutations observed within active site region, homology derived model was constructed using swiss-pdb viewer. The residue variation observed was with respect to Tyr347 in H5N1 versus Asn344 in H1N1 neuraminidase sequence, which resulted in geometrical modification of ligand binding domain.
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Download to read the full chapter text
Chapter PDF
References
Olsen, C.W., Brown, I.H., Easterday, B.C., Reeth, K.V.: Diseases of swine By Straw, B.E., Taylor, D.J., Swine Influenza. ch. 28 , pp. 469–470
AlKhawaja, S.: Consultant, Infectious Disease Physician. Ministry of Health Kingdom of Bahrain Medical Bulletin 31(2), 1–4 (2009)
Brown, D.: System set up after SARS epidemic was slow to alert global authorities (2009), http://www.washingtonpost.com/wp-dyn/content/article/2009/04/29/AR2009042904911.html
Chiu, S.S., Lau, Y.L., Chan, K.H., Wong, W.H.S., Peiris, J.S.M.: Influenza-related hospi-talizations among children in HongKong. N. Engl. J. Med. 347, 2097–2103 (2002)
Monto, A.S.: The role of antivirals in the control of influenza. Vaccine 21, 1796–1800 (2003)
Knox, C., Law, V., Jewison, T., Liu, P., Ly, S., Frolkis, A., Pon, A., Banco, K., Mak, C., Neveu, V., Djoumbou, Y., Eis-ner, R., Guo, A.C., Wishart, D.S.: DrugBank 3.0: a comprehensive resource for ’omics’ research on drugs. Nucleic Acids Res. 39(Database issue), D1035–D1041 (2011)
Berman, H.M., Westbrook, J., Feng, Z., Gilliland, G., Bhat, T.N., Weissig, H., Shindyalov, I.N., Bourne, P.E.: The Protein Data Bank. Nucleic Acids Res. 28, 235–242 (2000)
Jones, G., Willett, P., Glen, R.C.: Molecular recognition of receptor sites using a genetic algorithm with a description of desolvation. J. Mol. Biol. 245, 43–53 (1995)
Schneidman-Duhovny, D., Inbar, Y., Nussinov, R., Wolfson, H.J.: PatchDock and SymmDock: servers for rigid and symmetric docking. Nucl. Acids. Res. 33, W363–W367 (2005)
Chang, D.T.-H., Oyang, Y.-J., Lin, J.-H.: MEDock: a web server for efficient prediction of ligand binding sites based on a novel optimization algorithm. Nucleic Acids Research 33(suppl. 2), W233–W238
Schames, J.R., Henchman, R.H., Siegel, J.S., Sotriffer, C.A., Ni, H., McCammon, J.A.: Discovery of a novel binding trench in HIV integrase. J. Med. Chem. 47(8), 1879–1881 (2004)
Kitchen, D.B., Decornez, H., Furr, J.R., Bajorath, J.: Nat. Rev. Drug. Discov. 3, 935–949 (2004)
Wang, R., Lu, Y., Wang, S.: J. Med. Chem. 46, 2287–2303 (2003)
Charifson, P.S., Corkery, J.J., Murcko, M.A., Walters, W.P.: J. Med. Chem. 42, 5100–5109 (1999)
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer India
About this paper
Cite this paper
Nageswara Rao, G., Srinivasarao, P., Apparao, A., Rama Krishna Rao, T.K. (2013). Variation in Active Site Amino Residues of H1N1 Swine Flu Neuraminidase. In: Kumar M., A., R., S., Kumar, T. (eds) Proceedings of International Conference on Advances in Computing. Advances in Intelligent Systems and Computing, vol 174. Springer, New Delhi. https://doi.org/10.1007/978-81-322-0740-5_68
Download citation
DOI: https://doi.org/10.1007/978-81-322-0740-5_68
Publisher Name: Springer, New Delhi
Print ISBN: 978-81-322-0739-9
Online ISBN: 978-81-322-0740-5
eBook Packages: EngineeringEngineering (R0)