Abstract
Matrix metalloproteinase (MMP)-2 can degrade type IV collagen, and MMP-14 can activate pro-MMP-2. Bone sialoprotein (BSP) specifically binds pro-MMP-2 and active MMP-2. The expression of MMP-2, MMP-14, and BSP were analyzed by immunohistochemistry, in situ hybridization, and RT-PCR at the interface between cells of the epithelial rests of Malassez (ERM) and fibroblasts from human periodontal ligament (HPDL). ERM cells at the interface strongly expressed MMP-2 and MMP-14 proteins. In situ hybridization analysis showed that HPDL fibroblasts expressed MMP-2 mRNA, and ERM cells expressed MMP-14 mRNA at the interface strongly. BSP and its mRNA were expressed strongly in HPDL fibroblasts at the interface. RT-PCR analysis demonstrated that the expressions of MMP-2 mRNA and BSP mRNA were significantly high. These findings indicate that upregulated MMP-2 activated by MMP-14 in ERM cells and BSP in HPDL fibroblasts could degrade matrix molecules.
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References
Shimonishi M, Sato J, Takahashi N et al (2005) Expression of type IV collagen and laminin at the interface between epithelial cells and fibroblasts from human periodontal ligament. Eur J Oral Sci 113:34–40
Werb Z (1997) ECM and cell surface proteolysis: regulating cellular ecology. Cell 91:439–442
Acknowledgment
This work was supported by a Grant-in-Aid for Scientific Research (C) (No. 19592167) from Japan Society for the Promotion of Science, Japan.
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Shimonishi, M., Takahashi, I., Komatsu, M., Kikuchi, M. (2010). Activation of matrix metalloproteinase-2 at the interface between epithelial cells and fibroblasts from human periodontal ligament. In: Sasano, T., Suzuki, O. (eds) Interface Oral Health Science 2009. Springer, Tokyo. https://doi.org/10.1007/978-4-431-99644-6_19
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DOI: https://doi.org/10.1007/978-4-431-99644-6_19
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-99643-9
Online ISBN: 978-4-431-99644-6
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