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Reconstructing the Repertoire of Mite Allergens by Recombinant DNA Technology

  • Seow Theng Ong
  • Fook Tim Chew
Part of the Allergy Frontiers book series (ALLERGY, volume 6)

Abstract

House dust mites are a major source of inhalant allergens worldwide. More than 20 groups of house dust mite allergens have been officially recognized and listed in the World Health Organization – International Union of Immunological Societies (WHO/IUIS) list of allergens, (www.allergen.org). Our recent studies however show more than 40 groups may be present in the various species of dust mites with allergenic specificities varying from one to the other. One key feature of what is observed from the identification of these allergens is the large redundancy of what we can call an “allergen” across different species of mites as well as across diverse organisms. In this article, we also discuss the role and limitations of recombinant allergens particularly the expression systems used to produce these allergens and the difficulty in re-folding selected number of these proteins appropriately. Nevertheless, recombinant allergens have been essential in our understanding of the primary structures of these antigens, their epitopes and immunological properties.

Keywords

Allergy Clin Immunol House Dust Mite Allergen Recombinant Allergen House Dust Mite Allergen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer 2010

Authors and Affiliations

  • Seow Theng Ong
    • 1
  • Fook Tim Chew
    • 1
  1. 1.Department of Biological SciencesNational University of Singapore, Allergy and Molecular Immunology Laboratory, Lee Hiok Kwee Functional Genomics Laboratories, 14Singapore

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