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Flow-Assisted Analysis of Basophils: A Valuable Instrument for In Vitro Allergy Diagnosis

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Allergy Frontiers: Diagnosis and Health Economics

Part of the book series: Allergy Frontiers ((ALLERGY,volume 4))

It is clear that the basophil activation test (BAT) that rests upon flow-assisted quantification of in vitro-activated basophils provides the physician with a novel diagnostic tool that could rapidly spread because of the numerous flow cytometers already set in clinical and experimental laboratories.

Nowadays, the technique has been adopted for the diagnosis of inhalant allergy, natural rubber latex allergy, primary and secondary food allergies, hymenoptera venoms allergy and allergy for certain drugs. Applying donor basophils, the technique has proven to be useful in the detection of auto-antibodies responsible for chronic autoimmune urticaria. As the technique closely resembles the in vivo pathway ultimately leading to symptoms, it has already been successfully adopted for other objectives such as differentiation between clinically relevant and irrelevant IgE antibody results, quantitative evaluation of (residual) allergenicity of recombinant proteins and allergoids, component resolved diagnosis, to assess efficacy of anti-IgE treatment, to select and monitor specific immunotherapy and to study signalling in basophils. Finally, because basophils can be stimulated ex vivo, they provide the theoretical potential of measuring more than specific IgE, but rather a biological response from cells that were, until shortly before the assay, subject to the host's milieu.

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  1. Immunology — Allergology — Rheumatology, Campus Drie Eiken, University of Antwerp, Faculty of Medicine, Universiteitsplein 1, Antwerpen, B-2610, Belgium

    Didier G. Ebo & Chris H. Bridts

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  1. Didier G. Ebo
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  2. Chris H. Bridts
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  1. Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo, Japan

    Ruby Pawankar

  2. Southampton General Hospital, University of Southampton, Tremona Road, Southampton, UK

    Stephen T. Holgate

  3. UMKC School of Medicine, Children's Mercy Hospitals and Clinics, 2401 Gillham Road, Kansas City, MO, 64108, USA

    Lanny J. Rosenwasser

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Ebo, D.G., Bridts, C.H. (2009). Flow-Assisted Analysis of Basophils: A Valuable Instrument for In Vitro Allergy Diagnosis. In: Pawankar, R., Holgate, S.T., Rosenwasser, L.J. (eds) Allergy Frontiers: Diagnosis and Health Economics. Allergy Frontiers, vol 4. Springer, Tokyo. https://doi.org/10.1007/978-4-431-98349-1_11

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