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CD203c for Allergy Diagnosis

  • Vanessa G. Brown
  • Madeleine Ennis
Part of the Allergy Frontiers book series (ALLERGY, volume 4)

Limitations of using allergy diagnostic tests such as clinical data, skin tests, IgE and histamine measurements in all patients and situations has led to the introduction of more specific methods using flow cytometry to measure allergen-dependent activation of basophils. Such methods include the use of the CD63- and the more recently identified CD203c-activation assay. CD63 is associated with degranulating basophils and CD203c expression is found on activated/primed basophils. Both assays have proved to be potentially useful tools. CD63 has been used more extensively in a large number of clinical studies in the diagnosis of numerous allergens and whether the use of CD203c can improve the sensitivity, specificity, and positive and negative predictive values which have been established in the CD63 assay remains to be determined. This review will describe both the CD63 and CD203c flow cytometry-based techniques in allergy diagnosis and compare and address the important technical issues associated with each of the techniques. Additional studies using CD203c are required with different allergens, providing suitable thresholds for each allergen, so that this technique can be fully standardized and potentially become a reliable diagnostic test.

Keywords

Allergy Clin Immunol Basophil Activation Human Basophil Basophil Activation Test Allergy Diagnosis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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© Springer 2009

Authors and Affiliations

  • Vanessa G. Brown
    • 1
  • Madeleine Ennis
    • 1
  1. 1.Respiratory Research Group, Centre for Infection and ImmunitySchool of Medicine and Dentistry, Queen's University BelfastBelfastUK

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