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CD203c for Allergy Diagnosis

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Allergy Frontiers: Diagnosis and Health Economics

Part of the book series: Allergy Frontiers ((ALLERGY,volume 4))

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Limitations of using allergy diagnostic tests such as clinical data, skin tests, IgE and histamine measurements in all patients and situations has led to the introduction of more specific methods using flow cytometry to measure allergen-dependent activation of basophils. Such methods include the use of the CD63- and the more recently identified CD203c-activation assay. CD63 is associated with degranulating basophils and CD203c expression is found on activated/primed basophils. Both assays have proved to be potentially useful tools. CD63 has been used more extensively in a large number of clinical studies in the diagnosis of numerous allergens and whether the use of CD203c can improve the sensitivity, specificity, and positive and negative predictive values which have been established in the CD63 assay remains to be determined. This review will describe both the CD63 and CD203c flow cytometry-based techniques in allergy diagnosis and compare and address the important technical issues associated with each of the techniques. Additional studies using CD203c are required with different allergens, providing suitable thresholds for each allergen, so that this technique can be fully standardized and potentially become a reliable diagnostic test.

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References

  1. Dahinden CA, Kurimoto J, Baggiolini M, Dewald B, Walz A (1989) Histamine and sulfido-leukotriene release from human basophils: different effects of antigen, anti-IgE, C5a, f-Met-Leu-Phe and the novel neutrophil-activating peptide NAF. Int Arch Allergy Appl Immunol 90:113–118.

    PubMed  CAS  Google Scholar 

  2. Dvorak AM. (1998) A role for vesicles in human basophil secretion. Cell Tissue Res. 293:1–22.

    Article  PubMed  CAS  Google Scholar 

  3. Naclerio RM, Proud D, Togias AG, Adkinson NF, Jr, Meyers DA, Kagey-Sobotka A, Plaut M, Norman PS, Lichtenstein LM (1985) Inflammatory mediators in late antigen-induced rhinitis. N Engl J Med 313:65–70.

    PubMed  CAS  Google Scholar 

  4. Bascom R, Pipkorn U, Lichtenstein LM, Naclerio RM (1988) The influx of inflammatory cells into nasal washings during the late response to antigen challenge. Effect of systemic steroid pretreatment. Am Rev Respir Dis 138:406–412.

    PubMed  CAS  Google Scholar 

  5. Iliopoulos O, Baroody FM, Naclerio RM, Bochner BS, Kagey-Sobotka A, Lichtenstein LM (1992) Histamine-containing cells obtained from the nose hours after antigen challenge have functional and phenotypic characteristics of basophils. J Immunol 148:2223–2228.

    PubMed  CAS  Google Scholar 

  6. Guo CB, Liu MC, Galli SJ, Bochner BS, Kagey-Sobotka A, Lichtenstein LM (1994) Identification of IgE-bearing cells in the late-phase response to antigen in the lung as basophils. Am J Respir Cell Mol Biol 10:384–390.

    PubMed  CAS  Google Scholar 

  7. MacGlashan D, Jr, White JM, Huang SK, Ono SJ, Schroeder JT, Lichtenstein LM (1994) Secretion of IL-4 from human basophils. The relationship between IL-4 mRNA and protein in resting and stimulated basophils. J Immunol 152:3006–3016.

    CAS  Google Scholar 

  8. Gibbs BF, Haas H, Falcone FH, Albrecht C, Vollrath IB, Noll T, Wolff HH, Amon U (1996) Purified human peripheral blood basophils release interleukin-13 and preformed interleukin-4 following immunological activation. Eur J Immunol 26:2493–2498.

    Article  PubMed  CAS  Google Scholar 

  9. Li H, Sim TC, Alam R (1996) IL-13 released by and localized in human basophils. J Immunol 156:4833–4838.

    PubMed  CAS  Google Scholar 

  10. Pruzansky JJ, Patterson R (1988) Limiting concentrations of human basophil-bound IgE antibody required for histamine release Immunology 64:307–310.

    PubMed  CAS  Google Scholar 

  11. Gilbert HS, Ornstein L (1975) Basophil counting with a new staining method using alcian blue. Blood 46:279–286.

    PubMed  CAS  Google Scholar 

  12. Gane P, Pecquet C, Crespeau H, Lambin P, Leynadier F, Rouger P (1995) Flow cytometric monitoring of allergen induced basophil activation. Cytometry 19:361–365.

    Article  PubMed  CAS  Google Scholar 

  13. Bochner BS, Sterbinsky SA, Knol EF, Katz BJ, Lichtenstein LM, MacGlashan DW Jr, Schleimer RP (1994) Function and expression of adhesion molecules on human basophils. J Allergy Clin Immunol 94:1157–1162.

    Article  PubMed  CAS  Google Scholar 

  14. Sainte-Laudy J (1998) Application of flow cytometry to the analysis of activation of human basophils. Immunologic validation of the method. Allerg Immunol (Paris) 30:41–43.

    CAS  Google Scholar 

  15. Sabbah A, Sainte-Laudy J (1996) Flow cytometry applied to the analysis of lymphocyte and basophil activation. ACI International 8/4:116–119.

    Google Scholar 

  16. Knol EF, Mul FP, Jansen H, Calafat J, Roos D (1991) Monitoring human basophil activation via CD63 monoclonal antibody 435. J Allergy Clin Immunol 88:328–338.

    Article  PubMed  CAS  Google Scholar 

  17. Sainte-Laudy J, Sabbah A, Vallon C, Guerin JC (1998) Analysis of anti-IgE and allergen induced human basophil activation by flow cytometry. Comparison with histamine release. Inflamm Res 47:401–408.

    Article  PubMed  CAS  Google Scholar 

  18. Knol EF, Mul FP, Jansen H, Calafat J, Roos D (1991) Monitoring human basophil activation via CD63 monoclonal antibody 435. J Allergy Clin Immunol 88:328–338.

    Article  PubMed  CAS  Google Scholar 

  19. Israels SJ, McMillan EM, Robertson C, Singhory S, McNicol A (1996) The lysosomal granule membrane protein, LAMP-2, is also present in platelet dense granule membranes. Thromb Haemost 75:623–629.

    PubMed  CAS  Google Scholar 

  20. Vischer UM, Wagner DD (1993) CD63 is a component of Weibel-Palade bodies of human endothelial cells. Blood 82:1184–1191.

    PubMed  CAS  Google Scholar 

  21. Koyama Y, Suzuki M, Yoshida T (1998) CD63, a member of tetraspan transmembrane protein family, induces cellular spreading by reaction with monoclonal antibody on substrata. Biochem Biophys Res Commun 246:841–846.

    Article  PubMed  CAS  Google Scholar 

  22. Kuijpers TW, Tool AT, van der Schoot CE, Ginsel LA, Onderwater JJ, Roos D, Verhoeven AJ (1991) Membrane surface antigen expression on neutrophils: a reappraisal of the use of surface markers for neutrophil activation. Blood 78:1105–1111.

    PubMed  CAS  Google Scholar 

  23. Sainte-Laudy J, Boumediene A, Touraine F, Orsel I, Brianchon C, Bonnaud F, Cogne M (2007) Use of both CD63 up regulation and IgE down regulation for the flow cytometric analysis of allergen induced basophil activation. Definition of an activation index. Inflamm Res 56:291–296.

    Article  PubMed  CAS  Google Scholar 

  24. Monneret G, Gutowski MC, Bienvenu J (1999) Detection of allergen-induced basophil activation by expression of CD63 antigen using a tricolour flow cytometric method. Clin Exp Immunol 115:393–396.

    Article  PubMed  CAS  Google Scholar 

  25. Moneret-Vautrin DA, Sainte-Laudy J, Kanny G, Fremont S (1999) Human basophil activation measured by CD63 expression and LTC4 release in IgE-mediated food allergy. Ann Allergy Asthma Immunol 82:33–40.

    Article  PubMed  CAS  Google Scholar 

  26. Erdmann SM, Heussen N, Moll-Slodowy S, Merk HF, Sachs B (2003) CD63 expression on basophils as a tool for the diagnosis of pollen-associated food allergy: sensitivity and specificity. Clin Exp Allergy 33:607–614.

    Article  PubMed  CAS  Google Scholar 

  27. Sainte-Laudy J, Sabbah A, Drouet M, Lauret MG, Loiry M (2000) Diagnosis of venom allergy by flow cytometry. Correlation with clinical history, skin tests, specific IgE, histamine and leukotriene C4 release. Clin Exp Allergy 30:1166–1171.

    Article  PubMed  CAS  Google Scholar 

  28. Lambert C, Guilloux L, Dzviga C, Gourgaud-Massias C, Genin C (2003) Flow cytometry versus histamine release analysis of in vitro basophil degranulation in allergy to Hymenoptera venom. Cytometry B Clin Cytom 52:13–19.

    Article  PubMed  CAS  Google Scholar 

  29. Eberlein-Konig B, Rakoski J, Behrendt H, Ring J (2004) Use of CD63 expression as marker of in vitro basophil activation in identifying the culprit in insect venom allergy. J Investig Allergol Clin Immunol 14:10–16.

    PubMed  CAS  Google Scholar 

  30. Erdmann SM, Sachs B, Kwiecien R, Moll-Slodowy S, Sauer I, Merk HF (2004) The basophil activation test in wasp venom allergy: sensitivity, specificity and monitoring specific immu-notherapy. Allergy 59:1102–1109.

    Article  PubMed  CAS  Google Scholar 

  31. Sturm GJ, Bohm E, Trummer M, Weiglhofer I, Heinemann A, Aberer W (2004) The CD63 basophil activation test in Hymenoptera venom allergy: a prospective study. Allergy 59:1110–1117.

    Article  PubMed  CAS  Google Scholar 

  32. Cozon G, Ferrandiz J, Peyramond D, Brunet J (1999) Detection of activated basophils using flow cytometry for diagnosis in atopic patients. Allergol Immunopathol (Madr) 27:182–187.

    CAS  Google Scholar 

  33. Sanz ML, Sanchez G, Gamboa PM, Vila L, Uasuf C, Chazot M, Dieguez I, De Weck AL (2001) Allergen-induced basophil activation: CD63 cell expression detected by flow cytom-etry in patients allergic to Dermatophagoides pteronyssinus and Lolium perenne. Clin Exp Allergy 31:1007–1013.

    Article  PubMed  CAS  Google Scholar 

  34. Gonzalez-Munoz M, Villota J, Moneo I (2008) Analysis of basophil activation by flow cyto-metry in pediatric house dust mite allergy. Pediatr Allergy Immunol 19:342–347.

    Article  PubMed  Google Scholar 

  35. Paris-Kohler A, Demoly P, Persi L, Lebel B, Bousquet J, Arnoux B (2000) In vitro diagnosis of cypress pollen allergy by using cytofluorimetric analysis of basophils (Basotest). J Allergy Clin Immunol 105:339–345.

    Article  PubMed  CAS  Google Scholar 

  36. Ebo DG, Lechkar B, Schuerwegh AJ, Bridts CH, De Clerck LS, Stevens WJ (2002) Validation of a two-color flow cytometric assay detecting in vitro basophil activation for the diagnosis of IgE-mediated natural rubber latex allergy. Allergy 57:706–712.

    Article  PubMed  CAS  Google Scholar 

  37. Boumiza R, Monneret G, Forissier MF, Savoye J, Gutowski MC, Powell WS, Bienvenu J (2003) Marked improvement of the basophil activation test by detecting CD203c instead of CD63. Clin Exp Allergy 33:259–265.

    Article  PubMed  CAS  Google Scholar 

  38. Sanz ML, Gamboa PM, Garcia-Aviles C, Vila L, Dieguez I, Antepara I, de Weck AL (2003) Flow-cytometric cellular allergen stimulation test in latex allergy. Int Arch Allergy Immunol 130:33–39.

    Article  PubMed  CAS  Google Scholar 

  39. Hemery ML, Arnoux B, Dhivert-Donnadieu H, Rongier M, Barbotte E, Verdier R, Demoly P (2005) Confirmation of the diagnosis of natural rubber latex allergy by the Basotest method. Int Arch Allergy Immunol 136:53–57.

    Article  PubMed  CAS  Google Scholar 

  40. Abuaf N, Rajoely B, Ghazouani E, Levy DA, Pecquet C, Chabane H, Leynadier F (1999) Validation of a flow cytometric assay detecting in vitro basophil activation for the diagnosis of muscle relaxant allergy. J Allergy Clin Immunol 104:411–418.

    Article  PubMed  CAS  Google Scholar 

  41. Monneret G, Benoit Y, Debard AL, Gutowski MC, Topenot I, Bienvenu J (2002) Monitoring of basophil activation using CD63 and CCR3 in allergy to muscle relaxant drugs. Clin Immunol 102:192–199.

    Article  PubMed  CAS  Google Scholar 

  42. Sudheer PS, Hall JE, Read GF, Rowbottom AW, Williams PE (2005) Flow cytometric investigation of peri-anaesthetic anaphylaxis using CD63 and CD203c. Anaesthesia 60:251–256.

    Article  PubMed  CAS  Google Scholar 

  43. Sanz ML, Gamboa PM, Antepara I, Uasuf C, Vila L, Garcia-Aviles C, Chazot M, De Weck AL (2002) Flow cytometric basophil activation test by detection of CD63 expression in patients with immediate-type reactions to betalactam antibiotics. Clin Exp Allergy 32: 277–286.

    Article  PubMed  CAS  Google Scholar 

  44. Gamboa PM, Garcia-Aviles MC, Urrutia I, Antepara I, Esparza R, Sanz ML (2004) Basophil activation and sulfidoleukotriene production in patients with immediate allergy to betalactam antibiotics and negative skin tests. J Investig Allergol Clin Immunol 14:278–283.

    PubMed  CAS  Google Scholar 

  45. Torres MJ, Padial A, Mayorga C, Fernandez T, Sanchez-Sabate E, Cornejo-Garcia JA, Antunez C, Blanca M (2004) The diagnostic interpretation of basophil activation test in immediate allergic reactions to betalactams. Clin Exp Allergy 34:1768–1775.

    Article  PubMed  CAS  Google Scholar 

  46. Malbran A, Yeyati E, Rey GL, Galassi N (2007) Diclofenac induces basophil degranulation without increasing CD63 expression in sensitive patients. Clin Exp Immunol 147:99–105.

    PubMed  CAS  Google Scholar 

  47. Rodriguez-Trabado A, Camara-Hijon C, Ramos-Cantarino A, Porcel-Carreno SL, Jimenez-Timon S, Pereira-Navarro G, Hernandez-Arbeiza FJ, Fernandez-Pereira L (2008) Basophil activation test for the in vitro diagnosis of nonsteroidal anti-inflammatory drug hypersensitiv-ity. Allergy Asthma Proc 29:241–249.

    Article  PubMed  CAS  Google Scholar 

  48. Sainte-Laudy J, Vallon C, Guerin JC (1996) Diagnosis of latex allergy: comparison of hista-mine release and flow cytometric analysis of basophil activation. Inflamm Res 45(Suppl 1): S35–36.

    Article  PubMed  CAS  Google Scholar 

  49. Sainte-Laudy J, Le Provost A, Andre C, Vallon C (1995) Comparison of four methods for human basophil activation measurement: alcian blue staining, histamine and LTC4 release and flow cytometry. Proceedings of the XVI European Congress of Allergology and Clinical Immunology, pp. 257–260.

    Google Scholar 

  50. Aly Hassan IM, Crockard AD, Asghar MS, Edgar JD, Atkinson S (2001) Basotest and sux-amethonium allergy. Allergy 56:1016–1017.

    Article  PubMed  CAS  Google Scholar 

  51. Gane P, Pecquet C, Lambin P, Abuaf N, Leynadier F, Rouger P (1993) Flow cytometric evaluation of human basophils. Cytometry 14:344–348.

    Article  PubMed  CAS  Google Scholar 

  52. Boumiza R, Debard AL, Monneret G (2005) The basophil activation test by flow cytometry: recent developments in clinical studies, standardization and emerging perspectives. Clin Mol Allergy 3:9.

    Article  PubMed  CAS  Google Scholar 

  53. Ocmant A, Peignois Y, Mulier S, Hanssens L, Michils A, Schandene L (2007) Flow cytometry for basophil activation markers: the measurement of CD203c up-regulation is as reliable as CD63 expression in the diagnosis of cat allergy. J Immunol Methods 320:40–48.

    Article  PubMed  CAS  Google Scholar 

  54. Valent P, Besemer J, Muhm M, Majdic O, Lechner K, Bettelheim P (1989) Interleukin 3 activates human blood basophils via high-affinity binding sites. Proc Natl Acad Sci USA 86:5542–5546.

    Article  PubMed  CAS  Google Scholar 

  55. Gonzalez-Munoz M, Luque R, Nauwelaers F, Moneo I (2005) Detection of Anisakis simplex-induced basophil activation by flow cytometry. Cytometry B Clin Cytom 68:31–36.

    PubMed  Google Scholar 

  56. Ebo DG, Bridts CH, Hagendorens MM, Mertens CH, De Clerck LS, Stevens WJ (2006) Flow-assisted diagnostic management of anaphylaxis from rocuronium bromide. Allergy 61:935–939.

    Article  PubMed  CAS  Google Scholar 

  57. Varro R, Chen CH (2000) A no-wash 3-color basophil degranulation flow assay using the CD123 + HLA-DR- phenotype for basophil classification. Cytometry, Supplement 10:116.

    Google Scholar 

  58. Michova A, Abugalia M, Ivanova T, Nikolov G, Taskov H, Petrunov B (2006) Comparision of two-flow cytometry methods for basophil degranulation in patients sensitized to grass pollen. Allergy 61:1078–1083.

    Article  PubMed  CAS  Google Scholar 

  59. Aerts NE, Dombrecht EJ, Bridts CH, Hagendorens MM, de Clerck LS, Stevens WJ, Ebo DG (2008) Simultaneous flow cytometric detection of basophil activation marker CD63 and intracellular phosphorylated p38 mitogen-activated protein kinase in birch pollen allergy. Cytometry B Clin Cytom Aug 25. [Epub ahead of print].

    Google Scholar 

  60. Buhring HJ, Simmons PJ, Pudney M, Muller R, Jarrossay D, van Agthoven A, Willheim M, Brugger W, Valent P, Kanz L (1999) The monoclonal antibody 97A6 defines a novel surface antigen expressed on human basophils and their multipotent and unipotent progenitors. Blood 94:2343–2356.

    PubMed  CAS  Google Scholar 

  61. Buhring HJ, Seiffert M, Giesert C, Marxer A, Kanz L, Valent P, Sano K (2001) The basophil activation marker defined by antibody 97A6 is identical to the ectonucleotide pyrophos-phatase/phosphodiesterase 3. Blood 97:3303–3305.

    Article  PubMed  CAS  Google Scholar 

  62. Hennersdorf F, Florian S, Jakob A, Baumgartner K, Sonneck K, Nordheim A, Biedermann T, Valent P, Buhring HJ (2005) Identification of CD13, CD107a, and CD164 as novel basophil-activation markers and dissection of two response patterns in time kinetics of IgE-dependent upregulation. Cell Res 15:325–335.

    Article  PubMed  CAS  Google Scholar 

  63. Hauswirth AW, Natter S, Ghannadan M, Majlesi Y, Schernthaner GH, Sperr WR, Buhring HJ, Valenta R, Valent P (2002) Recombinant allergens promote expression of CD203c on basophils in sensitized individuals. J Allergy Clin Immunol 110:102–109.

    Article  PubMed  CAS  Google Scholar 

  64. Kahlert H, Cromwell O, Fiebig H (2003) Measurement of basophil-activating capacity of grass pollen allergens, allergoids and hypoallergenic recombinant derivatives by flow cytom-etry using anti-CD203c. Clin Exp Allergy 33:1266–1272.

    Article  PubMed  CAS  Google Scholar 

  65. Eberlein-Konig B, Varga R, Mempel M, Darsow U, Behrendt H, Ring J (2006) Comparison of basophil activation tests using CD63 or CD203c expression in patients with insect venom allergy. Allergy 61:1084–1085.

    Article  PubMed  CAS  Google Scholar 

  66. Abuaf N, Rostane H, Rajoely B, Gaouar H, Autegarden JE, Leynadier F, Girot R (2008) Comparison of two basophil activation markers CD63 and CD203c in the diagnosis of amoxi-cillin allergy. Clin Exp Allergy 38:921–928.

    Article  PubMed  CAS  Google Scholar 

  67. Platz IJ, Binder M, Marxer A, Lischka G, Valent P, Buhring HJ (2001) Hymenoptera-venom-induced upregulation of the basophil activation marker ecto-nucleotide pyrophosphatase/ phosphodiesterase 3 in sensitized individuals. Int Arch Allergy Immunol 126:335–342.

    Article  PubMed  CAS  Google Scholar 

  68. de Weck AL, Sanz ML (2003) For allergy diagnostic flow cytometry, detection of CD203c instead of CD63 is not at all an improvement in other hands. Clin Exp Allergy 33:849–52.

    Article  PubMed  Google Scholar 

  69. Ebo DG, Lechkar B, Schuerwegh AJ, Bridts CH, De Clerck LS, Stevens WJ (2003) Comments regarding ‘Marked improvement of the basophil activation test by detecting CD203c instead of CD63’′ by Boumiza et al. Clin Exp Allergy 33:849; author reply 852–853.

    Article  Google Scholar 

  70. Hauswirth AW, Sonneck K, Florian S, Krauth MT, Bohm A, Sperr WR, Valenta R, Schernthaner GH, Printz D, Fritsch G, Buhring HJ, Valent P (2007) Interleukin-3 promotes the expression of E-NPP3/CD203C on human blood basophils in healthy subjects and in patients with birch pollen allergy. Int J Immunopathol Pharmacol 20:267–278.

    PubMed  CAS  Google Scholar 

  71. Valent P, Hauswirth AW, Natter S, Sperr WR, Buhring HJ, Valenta R (2004) Assays for measuring in vitro basophil activation induced by recombinant allergens. Methods 32:265–270.

    Article  PubMed  CAS  Google Scholar 

  72. Marone G, Poto S, Giugliano R, Bonini S (1985) Studies on human basophil releasability. Int Arch Allergy Appl Immunol 77:103–106.

    PubMed  CAS  Google Scholar 

  73. Nguyen KL, Gillis S, MacGlashan DW, Jr (1990) A comparative study of releasing and nonreleasing human basophils: nonreleasing basophils lack an early component of the signal transduction pathway that follows IgE cross-linking. J Allergy Clin Immunol 85:1020–1029.

    Article  PubMed  CAS  Google Scholar 

  74. Binder M, Fierlbeck G, King T, Valent P, Buhring HJ (2002) Individual hymenoptera venom compounds induce upregulation of the basophil activation marker ectonucleotide pyrophos-phatase/phosphodiesterase 3 (CD203c) in sensitized patients. Int Arch Allergy Immunol 129:160–168.

    Article  PubMed  CAS  Google Scholar 

  75. Buhring HJ, Streble A, Valent P (2004) The basophil-specific ectoenzyme E-NPP3 (CD203c) as a marker for cell activation and allergy diagnosis. Int Arch Allergy Immunol 133:317–329.

    Article  PubMed  CAS  Google Scholar 

  76. De Week AL, Sanz ML, Gamboa PM, Aberer W, Bienvenu J, Blanca M, Demoly P, Ebo DG, Mayorga L, Monneret G, Sainte Laudy J (2008) Diagnostic tests based on human basophils: more potentials and perspectives than pitfalls. II. Technical issues. J Investig Allergol Clin Immunol 18:143–155.

    PubMed  Google Scholar 

  77. MacGlashan DW, Jr (1995) Graded changes in the response of individual human basophils to stimulation: distributional behavior of events temporally coincident with degranulation. J Leukoc Biol 58:177–188.

    PubMed  CAS  Google Scholar 

  78. Gober LM, Eckman JA, Sterba PM, Vasagar K, Schroeder JT, Golden DB, Saini SS (2007) Expression of activation markers on basophils in a controlled model of anaphylaxis. J Allergy Clin Immunol 119:1181–1188.

    Article  PubMed  CAS  Google Scholar 

  79. Valenta R, Lidholm J, Niederberger V, Hayek B, Kraft D, Gronlund H (1999) The recom-binant allergen-based concept of component-resolved diagnostics and immunotherapy (CRD and CRIT). Clin Exp Allergy 29:896–904.

    Article  PubMed  CAS  Google Scholar 

  80. Ebo DG, Hagendorens MM, Bridts CH, Schuerwegh AJ, De Clerck LS, Stevens WJ (2005) Flow cytometric analysis of in vitro activated basophils, specific IgE and skin tests in the diagnosis of pollen-associated food allergy. Cytometry B Clin Cytom 64:28–33.

    PubMed  Google Scholar 

  81. Erdmann SM, Sachs B, Schmidt A, Merk HF, Scheiner O, Moll-Slodowy S, Sauer I, Kwiecien R, Maderegger B, Hoffmann-Sommergruber K (2005) In vitro analysis of birch-pollen-associated food allergy by use of recombinant allergens in the basophil activation test. Int Arch Allergy Immunol 136:230–238.

    Article  PubMed  CAS  Google Scholar 

  82. Gamboa PM, Sanz ML, Caballero MR, Antepara I, Urrutia I, Jauregui I, Gonzalez G, Dieguez I, De Weck AL (2003) Use of CD63 expression as a marker of in vitro basophil activation and leukotriene determination in metamizol allergic patients. Allergy 58:312–317.

    Article  PubMed  CAS  Google Scholar 

  83. Gamboa P, Sanz ML, Caballero MR, Urrutia I, Antepara I, Esparza R, de Weck AL (2004) The flow-cytometric determination of basophil activation induced by aspirin and other non-steroidal anti-inflammatory drugs (NSAIDs) is useful for in vitro diagnosis of the NSAID hypersensitivity syndrome. Clin Exp Allergy 34:1448–1457.

    Article  PubMed  CAS  Google Scholar 

  84. Sanz ML, Maselli JP, Gamboa PM, Oehling A, Dieguez I, de Weck AL (2002) Flow cyto-metric basophil activation test: a review. J Investig Allergol Clin Immunol 12:143–154.

    PubMed  Google Scholar 

  85. Cesario TC, Slater L, Poo WJ, Spindler B, Walter B, Gorse G, Carandang G (1986) The effect of hydrocortisone on the production of gamma-interferon and other lymphokines by human peripheral blood mononuclear cells. J Interferon Res 6:337–347.

    PubMed  CAS  Google Scholar 

  86. Eberlein-Konig B, Schmidt-Leidescher C, Rakoski J, Behrendt H, Ring J (2006) In vitro basophil activation using CD63 expression in patients with bee and wasp venom allergy. J Investig Allergol Clin Immunol 16:5–10.

    PubMed  CAS  Google Scholar 

  87. Daher S, Santos LM, Sole D, De Lima MG, Naspitz CK, Musatti CC (1995) Interleukin-4 and soluble CD23 serum levels in asthmatic atopic children. J Investig Allergol Clin Immunol 5:251–254.

    PubMed  CAS  Google Scholar 

  88. Byron KA, Varigos G, Wootton A (1992) Hydrocortisone inhibition of human interleukin-4. Immunology 77:624–626.

    PubMed  CAS  Google Scholar 

  89. Sainte-Laudy J, Boumediene A, Touraine F, Orsel I, Cogne M (2006) Analysis of IgE down regulation induced by basophil activation. Application to the diagnosis of muscle relaxant allergic hypersensitivity by flow cytometry. Inflamm Res 55 Suppl 1:S21–2.

    Article  PubMed  CAS  Google Scholar 

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  1. Respiratory Research Group, Centre for Infection and Immunity, School of Medicine and Dentistry, Queen's University Belfast, Belfast, BT12 6BN, UK

    Vanessa G. Brown & Madeleine Ennis

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  1. Vanessa G. Brown
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  1. Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo, Japan

    Ruby Pawankar

  2. Southampton General Hospital, University of Southampton, Tremona Road, Southampton, UK

    Stephen T. Holgate

  3. UMKC School of Medicine, Children's Mercy Hospitals and Clinics, 2401 Gillham Road, Kansas City, MO, 64108, USA

    Lanny J. Rosenwasser

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Brown, V.G., Ennis, M. (2009). CD203c for Allergy Diagnosis. In: Pawankar, R., Holgate, S.T., Rosenwasser, L.J. (eds) Allergy Frontiers: Diagnosis and Health Economics. Allergy Frontiers, vol 4. Springer, Tokyo. https://doi.org/10.1007/978-4-431-98349-1_10

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