Skip to main content
SpringerLink
Log in
Book cover

Medaka pp 49–58Cite as

Transposable Elements Tol1 and Tol2

  • Chapter

  • 914 Accesses

Abstract

Medaka harbors active DNA-based transposable elements in its genome. This feature is unique among vertebrates and has enabled a wide range of studies on DNA-based elements, specifically on such topics as their transposition mechanisms, their population dynamics, and their contribution to genome evolution as natural mutagens. Findings of particular significance include the rapid expansion of an element in the medaka genome, the elevation of the mutation rate of host genes, and the disappearance of elements after they have functioned as mutagens. In addition to these contributions to basic biology, active DNA-based elements from medaka have also contributed to the development of tools for genetic manipulations, such as gene transfer, mutagenesis, gene tagging, and promoter/enhancer trapping. These tools are now used in a wide range of vertebrates, including humans, mice, and zebrafish.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

References

  • Asakawa K, Suster ML, Mizusawa K, Nagayoshi S, Kotani T, Urasaki A, Kishimoto Y, Hibi M, Kawakami K (2008) Genetic dissection of neural circuits by Tol2 transposon-mediated Gal4 gene and enhancer trapping in zebrafish. Proc Natl Acad Sci USA 105:1255–1260

    Article  PubMed  CAS  Google Scholar 

  • Bingham PM, Kidwell MG, Rubin GM (1982) The molecular basis of P-M hybrid dysgenesis: the role of the P element, a P-strain-specific transposon family. Cell 29:995–1004

    Article  PubMed  CAS  Google Scholar 

  • Calvi BR, Hong TJ, Findley SD, Gelbart WM (1991) Evidence for a common evolutionary origin of inverted repeat transposons in Drosophila and plants: hobo, activator, and Tam3. Cell 66:465–471

    Article  PubMed  CAS  Google Scholar 

  • Fedoroff N, Wessler S, Shure M (1983) Isolation of the transposable maize controlling elements Ac and Ds. Cell 35:235–242

    Article  PubMed  CAS  Google Scholar 

  • Fraser MJ, Ciszczon T, Elick T, Bauser C (1996) Precise excision of TTAA-specific lepidopteran transposons piggyBac (IFP2) and tagalong (TFP3) from the baculovirus genome in cell lines from two species of Lepidoptera. Insect Mol Biol 5:141–151

    Article  PubMed  CAS  Google Scholar 

  • Iida A, Inagaki H, Suzuki M, Wakamatsu Y, Hori H, Koga A (2004) The tyrosinase gene of the i b albino mutant of the medaka fish carries a transposable element insertion in the promoter region. Pigment Cell Res 17:158–164

    Article  PubMed  CAS  Google Scholar 

  • Iida A, Takamatsu N, Hori H, Wakamatsu Y, Shimada A, Shima A, Koga A (2005) Reversion mutation of i b oculocutaneous albinism to wild-type pigmentation in medaka fish. Pigment Cell Res 18:382–384

    Article  PubMed  CAS  Google Scholar 

  • Inagaki H, Bessho Y, Koga A, Hori H (1994) Expression of the tyrosinase-encoding gene in a colorless melanophore mutant of the medaka fish, Oryzias latipes. Gene (Amst) 150:319–324

    Article  CAS  Google Scholar 

  • Ivics Z, Hackett PB, Plasterk RH, Izsvak Z (1997) Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells. Cell 91:501–510

    Article  PubMed  CAS  Google Scholar 

  • Kasahara M, Naruse K, Sasaki S et al (2007) The medaka draft genome and insights into vertebrate genome evolution. Nature (Lond) 447:714–719

    Article  CAS  Google Scholar 

  • Kawakami K (2007) Tol2: a versatile gene transfer vector in vertebrates. Genome Biol 8 suppl 1:S7

    Article  PubMed  Google Scholar 

  • Koga A, Hori H (1997) Albinism due to transposable element insertion in fish. Pigment Cell Res 10:377–381

    Article  PubMed  CAS  Google Scholar 

  • Koga A, Hori H (1999) Homogeneity in the structure of the medaka fish transposable element Tol2. Genet Res 73:7–14

    Article  PubMed  CAS  Google Scholar 

  • Koga A, Hori H (2000) Detection of de novo insertion of the medaka fish transposable element Tol2. Genetics 156:1243–1247

    PubMed  CAS  Google Scholar 

  • Koga A, Hori H (2001) The Tol2 transposable element of the medaka fish: an active DNA-based element naturally occurring in a vertebrate genome. Genes Genet Syst 76:1–8

    Article  PubMed  CAS  Google Scholar 

  • Koga A, Inagaki H, Bessho Y, Hori H (1995) Insertion of a novel transposable element in the tyrosinase gene is responsible for an albino mutation in the medaka fish, Oryzias latipes. Mol Gen Genet 249:400–405

    Article  PubMed  CAS  Google Scholar 

  • Koga A, Suzuki M, Inagaki H, Bessho Y, Hori H (1996) Transposable element in fish. Nature (Lond) 383:30

    Article  CAS  Google Scholar 

  • Koga A, Suzuki M, Maruyama Y, Tsutsumi M, Hori H (1999) Amino acid sequence of a putative transposase protein of the medaka fish transposable element Tol2 deduced from mRNA nucleotide sequences. FEBS Lett 461:295–298

    Article  PubMed  CAS  Google Scholar 

  • Koga A, Shimada A, Shima A, Sakaizumi M, Tachida H, Hori H (2000) Evidence for recent invasion of the medaka fish genome by the Tol2 transposable element. Genetics 155:273–281

    PubMed  CAS  Google Scholar 

  • Koga A, Hori H, Sakaizumi M (2002a) Gene transfer and cloning of flanking chromosomal regions using the medaka fish Tol2 transposable element. Mar Biotechnol 4:6–11

    Article  PubMed  CAS  Google Scholar 

  • Koga A, Sakaizumi M, Hori H (2002b) Transposable elements in medaka fish. Zool Sci 19:1–6

    Article  PubMed  CAS  Google Scholar 

  • Koga A, Iida A, Hori H, Shimada A, Shima A (2006) Vertebrate DNA transposon as a natural mutator: the medaka fish Tol2 element contributes to genetic variation without recognizable traces. Mol Biol Evol 23:1414–1419

    Article  PubMed  CAS  Google Scholar 

  • Koga A, Shimada A, Kuroki T, Hori H, Kusumi J, Kyono-Hamaguchi Y, Hamaguchi S (2007a) The Tol1 transposable element of the medaka fish moves in human and mouse cells. J Hum Genet 52:628–635

    Article  PubMed  CAS  Google Scholar 

  • Koga A, Higashide I, Hori H, Wakamatsu Y, Kyono-Hamaguchi Y, Hamaguchi S (2007b) The Tol1 element of medaka fish is transposed with only terminal regions and can deliver large DNA fragments into the chromosomes. J Hum Genet 52:1026–1030

    Article  PubMed  CAS  Google Scholar 

  • Koga A, Cheah FS, Hamaguchi S, Yeo GH, Chong SS (2008) Germline transgenesis of zebrafish using the medaka Tol1 transposon system. Dev Dyn 237:2466–2474

    Article  PubMed  CAS  Google Scholar 

  • Korzh V (2007) Transposons as tools for enhancer trap screens in vertebrates. Genome Biol 8 suppl 1:S8

    Article  PubMed  Google Scholar 

  • Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Parant JM, Yost HJ, Kanki JP, Chien CB (2007) The Tol2kit: a multisite gateway-based construction kit for Tol2 transposon transgenesis constructs. Dev Dyn 236:3088–3099

    Article  PubMed  CAS  Google Scholar 

  • Naruse K (1996) Classification and phylogeny of fishes of the genus Oryzias. Fish Biol J Medaka 8:1–10

    Google Scholar 

  • Nitasaka E, Yamazaki T (1994) The relationship between DNA structural variation and activities of P elements in P and Q strains of Drosophila melanogaster. Heredity 73:608–615

    Article  PubMed  CAS  Google Scholar 

  • Pace JK 2nd, Gilbert C, Clark MS (2008) Repeated horizontal transfer of a DNA transposon in mammals and other tetrapods. Proc Natl Acad Sci USA 105:17023–17028

    Article  PubMed  CAS  Google Scholar 

  • Rubin GM, Kidwell MG, Bingham PM (1982) The molecular basis of P-M hybrid dysgenesis: the nature of induced mutations. Cell 29:987–994

    Article  PubMed  CAS  Google Scholar 

  • Sakaizumi M, Uwa H, Jeon S-R (1987) Genetic diversity of the East Asian populations of the freshwater fish, Oryzias. Zool Sci 4:1003

    Google Scholar 

  • Takehana Y, Naruse K, Sakaizumi M (2005) Molecular phylogeny of the medaka fishes genus Oryzias (Beloniformes: Adrianichthyidae) based on nuclear and mitochondrial DNA sequences. Mol Phylogenet Evol 36:417–428

    Article  PubMed  CAS  Google Scholar 

  • Tomita H (1975) Mutant genes in the medaka. In: Yamamoto T (ed) Medaka (killifish): biology and strains. Yugakusha, Tokyo, pp 251–272

    Google Scholar 

  • Urasaki A, Asakawa K, Kawakami K (2008) Efficient transposition of the Tol2 transposable element from a single-copy donor in zebrafish. Proc Natl Acad Sci USA 105:19827–19832

    Article  PubMed  CAS  Google Scholar 

  • Villefranc JA, Amigo J, Lawson ND (2007) Gateway compatible vectors for analysis of gene function in the zebrafish. Dev Dyn 236:3077–3087

    Article  PubMed  CAS  Google Scholar 

  • Yokoyama T, Silversides DW, Waymire KG, Kwon BS, Takeuchi T, Overbeek PA (1990) Conserved cysteine to serine mutation in tyrosinase is responsible for the classical albino mutation in laboratory mice. Nucleic Acids Res 18:7293–7298

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Primate Research Institute, Kyoto University, 41-2 Kanrin, Inuyama, Aichi, 484-8506, Japan

    Akihiko Koga

Authors
  1. Akihiko Koga
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Akihiko Koga .

Editor information

Editors and Affiliations

  1. Laboratory of Bioresources, National Institute for Basic Biology, 38 Nishigonaka, Myodaiji, Okazaki, Aichi, 444-8585, Japan

    Kiyoshi Naruse Ph.D. (Associate Professor) (Associate Professor)

  2. Laboratory of Molecular Genetics for Reproduction, National Institute for Basic Biology, 5-1 Higashiyama, Myodaiji, Okazaki, Aichi, 444-8787, Japan

    Minoru Tanaka Ph.D. (Associate Professor) (Associate Professor)

  3. Department of Biological Sciences Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan

    Hiroyuki Takeda Ph.D. (Professor) (Professor)

Rights and permissions

Reprints and permissions

Copyright information

© 2011 Springer

About this chapter

Cite this chapter

Koga, A. (2011). Transposable Elements Tol1 and Tol2 . In: Naruse, K., Tanaka, M., Takeda, H. (eds) Medaka. Springer, Tokyo. https://doi.org/10.1007/978-4-431-92691-7_4

Download citation

Publish with us

Policies and ethics

Search

Navigation