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Medaka pp 287-304 | Cite as

Interspecific Medaka Hybrids as Experimental Models for Investigating Cell Division and Germ Cell Development

  • Toshiharu Iwai
  • Chiharu Sakai
  • Fumiko Konno
  • Masakane Yamashita

Abstract

Interspecific hybrids in the genus Oryzias (medaka) usually have ­abnormalities in reproduction and development. Analysis of the causes of the abnormalities at cellular and molecular levels will contribute to the understanding of basic mechanisms of cell division and gametogenesis. We describe the molecular and cellular mechanisms of embryonic lethality in a hybrid between Oryzias latipes and Oryzias hubbsi and of sex-specific abnormal gametogenesis in a hybrid between O. latipes and Oryzias curvinotus. Chromosomes derived from O. hubbsi are selectively eliminated in both O. latipes (♀)–hubbsi (♂) and O. hubbsi (♀)–latipes (♂) embryos. We propose a possible cellular mechanism of the abnormal mitosis. We also suggest that the hybrid M-phase-promoting factor (MPF; complex of Cdc2 and cyclin B, each of which is derived from different species) is a general molecule causing defective cell divisions in hybrids. In a hybrid between O. latipes and O. curvinotus, spermiogenesis proceeds and sperm-like cells each having 4C DNA are produced without cytokinesis. In hybrid females, the majority of oocytes stop meiosis at the zygotene stage, although a small number of oocytes give rise to diploid eggs. An incomplete synaptonemal complex devoid of SCP1 is likely to cause the aberrant gametogenesis in both sexes in common.

Keywords

Hybrid Cell Synaptonemal Complex Metaphase Plate Spindle Pole Chromosome Elimination 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

CE

Central element

CGH

Comparative genomic hybridization

CPC

Chromosomal passenger complex

DAPI

4¢,6-Diamidino-2-phenylindole

FISH

Fluorescence in situ hybridization

INCENP

Inner centromere protein

LEs

Lateral elements

MBT

Midblastula transition

MPF

M-phase-promoting factor

SC

Synaptonemal complex

TFs

Transverse filaments

WCP

Whole chromosome painting

Notes

Acknowledgments

We are deeply grateful to the colleagues in our laboratory as well as in our collaborator’s laboratories for their contributions, especially Yohei Shimizu, Yoichi Matsuda, Asato Kuroiwa, Takashi Iwamatsu, Mitsuru Sakaizumi, Naoki Shibata, Koichi Mita, Fumio Kasai, and Hiroshi Mitani. This work was supported by funding to M.Y. from the Program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN), under the auspices of the Bio-Oriented Technology Research Advancement Institution (BRAIN).

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Copyright information

© Springer 2011

Authors and Affiliations

  • Toshiharu Iwai
    • 1
  • Chiharu Sakai
    • 2
  • Fumiko Konno
    • 3
  • Masakane Yamashita
    • 4
  1. 1.Laboratory of Reproductive and Developmental Biology, Faculty of ScienceHokkaido UniversitySapporoJapan
  2. 2.Laboratory of Reproductive and Developmental Biology, Faculty of ScienceHokkaido UniversitySapporoJapan
  3. 3.Laboratory of Reproductive and Developmental Biology, Faculty of ScienceHokkaido UniversitySapporoJapan
  4. 4.Laboratory of Reproductive and Developmental Biology, Faculty of ScienceHokkaido UniversitySapporoJapan

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