Establishment of Transplantable Biliary Cancer Lines
A transplantable cancer line from the gallbladder was established in Syrian golden hamsters. The primary tumor of the gallbladder was induced by a subcutaneous injection of N-nitrosobis(2-oxopropyl)amine (BOP) in hamsters after cholecystoduodenostomy with dissection of the common duct. The tumor was inoculated into the cavity of the gallbladder and subcutaneous tissue and was transplantable, with an uptake rate of 100%. Histologically, the primary induced gallbladder tumor was well-differentiated papillary adenocarcinoma and the principal structure of all allograft tumors was of poorly differentiated ductal adenocarcinoma. These tumors expressed blood group-related antigens, including A, H, and P-glycoproteins. To our knowledge, this is the first report of carcinoma of the gallbladder transplantable in a laboratory animal.
KeywordsGallbladder Carcinoma Cancer Line Tubular Adenocarcinoma Syrian Golden Hamster Avidin Biotin Peroxidase Complex
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