Establishment of Transplantable Biliary Cancer Lines

  • Tomohiro Fukahori
  • Keiji Inoue
  • Tsutomu Tomioka
  • Yoshitsugu Tajima
  • Tsukasa Tsunoda
  • Takashi Kanematsu

A transplantable cancer line from the gallbladder was established in Syrian golden hamsters. The primary tumor of the gallbladder was induced by a subcutaneous injection of N-nitrosobis(2-oxopropyl)amine (BOP) in hamsters after cholecystoduodenostomy with dissection of the common duct. The tumor was inoculated into the cavity of the gallbladder and subcutaneous tissue and was transplantable, with an uptake rate of 100%. Histologically, the primary induced gallbladder tumor was well-differentiated papillary adenocarcinoma and the principal structure of all allograft tumors was of poorly differentiated ductal adenocarcinoma. These tumors expressed blood group-related antigens, including A, H, and P-glycoproteins. To our knowledge, this is the first report of carcinoma of the gallbladder transplantable in a laboratory animal.


Gallbladder Carcinoma Cancer Line Tubular Adenocarcinoma Syrian Golden Hamster Avidin Biotin Peroxidase Complex 
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Copyright information

© Springer 2009

Authors and Affiliations

  • Tomohiro Fukahori
    • 1
  • Keiji Inoue
    • 2
  • Tsutomu Tomioka
    • 3
  • Yoshitsugu Tajima
    • Tsukasa Tsunoda
      • 4
    • Takashi Kanematsu
      1. 1.Department of SurgeryAino Memorial HospitalUnzenJapan
      2. 2.Department of SurgeryNagasaki Municipal HospitalNagasakiJapan
      3. 3.Department of SurgeryNagasaki Yurino HospitalNishisonogiJapan
      4. 4.Department of SurgeryDivision of Gastroenterological Surgery, Kawasaki Medical SchoolKurashikiJapan

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