Advertisement

Specific Features of Transient Ras and Sustained Rap1 Activation

  • Yu-ichi Ozaki
  • Shinsuke Uda
  • Shinya Kuroda
Conference paper

Epidermal growth factor (EGF) and nerve growth factor (NGF) induce transient and sustained extracellular signal-regulated kinase (ERK) activation, respectively, and regulate cell growth and differentiation in PC12 cells (Fig. 1a) (Gotoh et al. 1990; Qiu and Green 1992; Traverse et al. 1992; Vaudry et al. 2002). Transient and sustained ERK activation in PC12 cells depends on transient Ras activation and sustained Rap1 activation, respectively (Fig. 1b) (Vaudry et al. 2002; York et al. 1998). We have previously modeled EGF- and NGF-dependent ERK activation in PC12 cells, and found that transient Ras and consequent ERK activation depends on rapidly increasing rates of EGF and NGF, whereas sustained Rap1 and consequent ERK activation depends on the final concentration of NGF (Sasagawa et al. 2005). We here construct simple Ras and Rap1 models whose inactivation is dependent on and independent of stimulation, respectively (Fig. 1c), and summarize how the modes of inactivation of Ras and Rap1 makes the features distinct.

Keywords

Epidermal Growth Factor PC12 Cell Nerve Growth Factor Transient Peak Step Stimulation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Gotoh Y, Nishida E, Yamashita T et al (1990) Microtubule-associated-protein (MAP) kinase activated by nerve growth factor and epidermal growth factor in PC12 cells. Identity with the mitogen-activated MAP kinase of fibroblastic cells. Eur J Biochem 193:661–669.PubMedCrossRefGoogle Scholar
  2. Qiu MS, Green SH (1992) PC12 cell neuronal differentiation is associated with prolonged p21ras activity and consequent prolonged ERK activity. Neuron 9:705–717.CrossRefGoogle Scholar
  3. Sasagawa S, Ozaki Y, Fujita K et al (2005) Prediction and validation of the distinct dynamics of transient and sustained ERK activation. Nat Cell Biol 7:365–373.PubMedCrossRefGoogle Scholar
  4. Traverse S, Gomez N, Paterson H et al (1992) Sustained activation of the mitogen-activated protein (MAP) kinase cascade may be required for differentiation of PC12 cells. Comparison of the effects of nerve growth factor and epidermal growth factor. Biochem J 288(pt 2):351–355.PubMedGoogle Scholar
  5. Vaudry D, Stork PJ, Lazarovici P et al (2002) Signaling pathways for PC12 cell differentiation: making the right connections. Science 296:1648–1649.PubMedCrossRefGoogle Scholar
  6. York RD, Yao H, Dillon T et al (1998) Rap1 mediates sustained MAP kinase activation induced by nerve growth factor. Nature (Lond) 392:622–666.CrossRefGoogle Scholar

Copyright information

© Springer 2009

Authors and Affiliations

  • Yu-ichi Ozaki
    • 1
  • Shinsuke Uda
    • 1
  • Shinya Kuroda
    • 1
  1. 1.Department of Biophysics and Biochemistry, Graduate School of ScienceUniversity of Tokyo, CREST, Japan Science and Technology AgencyBunkyo-kuJapan

Personalised recommendations