Analysis of Oligosaccharides by Capillary Electrophoresis

  • Mitsuhiro Kinoshita
  • Kazuaki Kakehi


Carbohydrate analysis has a special feature which should be capable of high-resolution separation, because oligosaccharides derived from glycoconjugates are usually composed of a complex mixture of carbohydrates including isomers and homologues. Capillary electrophoresis (CE) is one of the most powerful techniques in terms of resolving power and is applied to the analysis of various carbohydrates from glycoconjugates. A combination of CE and laser-induced fluorescence (LIF) detection enables to detect even fmol (10−15 mol) to amol (10−18 mol) of carbohydrates. Multiplexed CE systems and multichannel microfluidic devices have been developed for high-throughput research and are applied to clinical analysis of serum glycome.


Hyaluronic Acid Capillary Electrophoresis Capillary Electrophoresis System Constant Voltage Mode Streptococcus Zooepidemicus 
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  1. Kakehi K, Honda S (1996) Analysis of glycoproteins, glycopeptides and glycoprotein-derived oligosaccharides by high-performance capillary electrophoresis. J Chromatogr A 720(1–2):377–393PubMedCrossRefGoogle Scholar
  2. Kakehi K, Kinoshita M, Hayase S, Oda Y (1999) Capillary electrophoresis of N-acetylneuraminic acid polymers and hyaluronic acid: correlation between migration order reversal and biological functions. Anal Chem 71(8):1592–1596PubMedCrossRefGoogle Scholar
  3. Kakehi K, Kinoshita M, Nakano M (2002) Analysis of glycoproteins and the oligosaccharides thereof by high-performance capillary electrophoresis-significance in regulatory studies on biopharmaceutical products. Biomed Chromatogr 16(2):103–115PubMedCrossRefGoogle Scholar
  4. Kamoda S, Nakanishi Y, Kinoshita M, Ishikawa R, Kakehi K (2006a) Analysis of glycoprotein-derived oligosaccharides in glycoproteins detected on two-dimensional gel by capillary electrophoresis using on-line concentration method. J Chromatogr A 1106(1–2):67–74PubMedCrossRefGoogle Scholar
  5. Kamoda S, Ishikawa R, Kakehi K (2006b) Capillary electrophoresis with laser-induced fluorescence detection for detailed studies on N-linked oligosaccharide profile of therapeutic recombinant monoclonal antibodies. J Chromatogr A 1133(1–2):332–339PubMedCrossRefGoogle Scholar

Copyright information

© Springer 2008

Authors and Affiliations

  • Mitsuhiro Kinoshita
    • 1
  • Kazuaki Kakehi
    • 1
  1. 1.Faculty of Pharmaceutical SciencesKinki UniversityHigashi, OsakaJapan

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