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Analysis of Oligosaccharides by Capillary Electrophoresis

  • Mitsuhiro Kinoshita
  • Kazuaki Kakehi

Abstract

Carbohydrate analysis has a special feature which should be capable of high-resolution separation, because oligosaccharides derived from glycoconjugates are usually composed of a complex mixture of carbohydrates including isomers and homologues. Capillary electrophoresis (CE) is one of the most powerful techniques in terms of resolving power and is applied to the analysis of various carbohydrates from glycoconjugates. A combination of CE and laser-induced fluorescence (LIF) detection enables to detect even fmol (10−15 mol) to amol (10−18 mol) of carbohydrates. Multiplexed CE systems and multichannel microfluidic devices have been developed for high-throughput research and are applied to clinical analysis of serum glycome.

Keywords

Hyaluronic Acid Capillary Electrophoresis Capillary Electrophoresis System Constant Voltage Mode Streptococcus Zooepidemicus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer 2008

Authors and Affiliations

  • Mitsuhiro Kinoshita
    • 1
  • Kazuaki Kakehi
    • 1
  1. 1.Faculty of Pharmaceutical SciencesKinki UniversityHigashi, OsakaJapan

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