Separation of Oligosaccharides by 2D HPLC

  • Shunji Natsuka


A method for separation and identification of fluorescence-tagged oligosaccharides by two kinds of chromatography was reported by Hase in 1978 (Hase et al. 1978). Since then the microanalysis of labeled glycans has been greatly improved through the use of HPLC, and a sensitivity of glycan detection at the femtomole level (10−15 mol) has been obtained.


Ammonium Formate Elution Position Femtomole Level Conversion Index Sample Glycan 
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  1. Hase S, Ikenaka T, Matsushima Y (1978) Structure analyses of oligosaccharides by tagging of the reducing end sugars with a fluorescent compound. Biochem Biophys Res Commun 85:257–263PubMedCrossRefGoogle Scholar
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Copyright information

© Springer 2008

Authors and Affiliations

  • Shunji Natsuka
    • 1
  1. 1.Department of Chemistry, Graduate School of ScienceOsaka UniversityToyonaka, OsakaJapan

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