Separation of Oligosaccharides by 2D HPLC

Natsuka, Shunji

doi:10.1007/978-4-431-77924-7_8

Separation of Oligosaccharides by 2D HPLC

Shunji Natsuka⁸

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Abstract

A method for separation and identification of fluorescence-tagged oligosaccharides by two kinds of chromatography was reported by Hase in 1978 (Hase et al. 1978). Since then the microanalysis of labeled glycans has been greatly improved through the use of HPLC, and a sensitivity of glycan detection at the femtomole level (10⁻¹⁵ mol) has been obtained.

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References

Hase S, Ikenaka T, Matsushima Y (1978) Structure analyses of oligosaccharides by tagging of the reducing end sugars with a fluorescent compound. Biochem Biophys Res Commun 85:257–263
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Authors and Affiliations

Department of Chemistry, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka, 560-0043, Japan
Shunji Natsuka

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Shunji Natsuka
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Editors and Affiliations

Department of Disease Glycomics, Research Institute for Microbial Diseases, Osaka University, 1-1 Yamadaoka, Suita, Osaka, 565-0871, Japan
Naoyuki Taniguchi M.D., Ph.D. (Professor) (Professor)
Systems Glycobiology Group, Advanced Science Institute, RIKEN, Wako, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan
Naoyuki Taniguchi M.D., Ph.D. (Professor) (Professor)
Institute of Glycotechnology, Future Science and Technology Joint Research Center, Tokai University, Hiratsuka, Kanagawa, 259-1292, Japan
Akemi Suzuki M.D., Ph.D. (Professor) (Professor)
RIKEN Discovery Research Institute, Synthetic Cellular Chemistry Laboratory, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan
Yukishige Ito Ph.D. (Chief Researcher) (Chief Researcher)
Research Center for Medical Glycoscience, Advanced Industrial Science and Technology, AIST Tsukuba, Central 2, Tsukuba, Ibaraki, 305-8568, Japan
Hisashi Narimatsu M.D., Ph.D.
Research Center for Glycobiotechnology, Ritsumeikan University, 1-1-1 Noji-Higashi, Kusatsu, Shiga, 525-8577, Japan
Toshisuke Kawasaki Ph.D. (Professor) (Professor)
Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka, 560-0043, Japan
Sumihiro Hase Ph.D. (Professor) (Professor)

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Natsuka, S. (2008). Separation of Oligosaccharides by 2D HPLC. In: Taniguchi, N., Suzuki, A., Ito, Y., Narimatsu, H., Kawasaki, T., Hase, S. (eds) Experimental Glycoscience. Springer, Tokyo. https://doi.org/10.1007/978-4-431-77924-7_8

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DOI: https://doi.org/10.1007/978-4-431-77924-7_8
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-77923-0
Online ISBN: 978-4-431-77924-7
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