Chemical Labeling of Sialyloligo/polymer

  • Chihiro Sato


In most cases, sialic acids are present as α2,3- or α2,6-linked monosialyl residues at the non-reducing terminal positions of glycan chains on glycoproteins and glycolipids. In rare cases, sialic acids are linked to each other to form a polymerized structure, di/oligo/polysialic acid (diSia/oligoSia/polySia). The polySia glycotope exhibits structural diversity in the sialic acid components (Neu5Ac, Neu5Gc, and KDN), in the intersialyl linkages (α2 → 5 Oglycolyl , α2 → 8, α2 → 9, and α2 → 8/9) and in the degree of polymerization (DP). Highly sensitive chemical methods to detect di/oligo/polysialic acids were developed (Sato et al. 1998, 1999) with a highly specific reagent for α-keto acid (Hara et al. 1987), and frequent di/oligo/polysialylation on glycoproteins were demonstrated (Sato 2004).


Sialic Acid Pyruvic Acid Sialic Acid Residue Anion Exchange Column Polysialic Acid 
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  3. Sato C, Inoue S, Matsuda T, Kitajima K (1998) Development of a highly sensitive chemical method for detecting α2 → 8-linked oligo/polysialic acid residues in glycoproteins blotted on the membrane. Anal Biochem 261:191–197PubMedCrossRefGoogle Scholar
  4. Sato C, Inoue S, Matsuda T, Kitajima K (1999) Fluorescent-assisted detection of oligosialyl units in glycoconjugates. Anal Biochem 266:102–109PubMedCrossRefGoogle Scholar

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© Springer 2008

Authors and Affiliations

  • Chihiro Sato
    • 1
  1. 1.Laboratory of Animal Cell Function, Bioscience and Biotechnology CenterNagoya UniversityNagoyaJapan

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