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Endoglycosidases (Glycosaminoglycans)

  • Ikuko Kakizaki
  • Keiichi Takagaki

Abstract

Currently established biotechnologies are mainly based on genetic engineering and protein engineering, and they provide a number of valuable recombinant proteins. These technologies have brought benefits to medicine by enabling the production of various drugs. However, genetically produced recombinant proteins often lack entirely or partially their native sugar chains or have sugar chains that are different from the original sugar chains, thus being apt to exert insufficient specific bioactivities or to be unstable. The cause of this lack of sugar chains of recombinant proteins is that, DNA contains genetic information only on amino acid sequences but not on the biosynthesis of sugar chains that link to proteins. This problem could be solved by technologies to attach sugar chains to sugar chain-deficient recombinant protein. Moreover, attaching artificially synthesized bioactive sugar chains to protein would make it possible to create glycoproteins with additional biological activity. We focused on the transglycosylation activity of endoglycosidases acting on proteoglycan and developed methods for the reconstruction of their sugar chains, glycosaminoglycan (GAG), and for attachment of GAGs to protein.

Keywords

Sugar Chain Disaccharide Unit Monosaccharide Residue Transglycosylation Activity Specific Bioactivity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Ishido K, Takagaki K, Iwafune M, Yoshihara S, Sasaki M, Endo M (2002) Enzymatic attachment of glycosaminoglycan chain to peptide using the sugar chain transfer reaction with endo-beta-xylosidase. J Biol Chem 277:11889–11895PubMedCrossRefGoogle Scholar
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Copyright information

© Springer 2008

Authors and Affiliations

  • Ikuko Kakizaki
    • 1
  • Keiichi Takagaki
    • 1
  1. 1.Department of BiochemistryHirosaki University School of MedicineHirosakiJapan

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