Alteration of N-Linked Oligosaccharides
Oligosaccharides are linked to the protein surface and play roles in a number of biological events. Therefore, much attention is being paid to research and investigate the function of the oligosaccharides. Oligosaccharides on glycoprotein are roughly divided into two groups: O-linked and N-linked types. The N-linked type is a large branched oligosaccharide, which is further divided into three types: complex, hybrid, and high-mannose type. All oligosaccharides are linked to the nitrogen of asparagine by an N-glycosyl linkage. In order to prove the function of N-glycan on the protein surface, a convenient synthetic method for N-glycan chain should be developed. However, the chemical synthesis is time-consuming due to repetitive protection/deprotection steps. N-Glycans in glycoprotein show structural diversity in its oligosaccharides, the so-called glycoform. Here, I would like to introduce recent synthetic developments focusing on diverse N-linked oligosaccharides based on semisynthesis.
KeywordsBenzyl Ester Pool Fraction Glycosidase Digestion Convenient Synthetic Method Individual Oligosaccharide
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