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Alteration of N-Linked Oligosaccharides

  • Yasuhiro Kajihara

Abstract

Oligosaccharides are linked to the protein surface and play roles in a number of biological events. Therefore, much attention is being paid to research and investigate the function of the oligosaccharides. Oligosaccharides on glycoprotein are roughly divided into two groups: O-linked and N-linked types. The N-linked type is a large branched oligosaccharide, which is further divided into three types: complex, hybrid, and high-mannose type. All oligosaccharides are linked to the nitrogen of asparagine by an N-glycosyl linkage. In order to prove the function of N-glycan on the protein surface, a convenient synthetic method for N-glycan chain should be developed. However, the chemical synthesis is time-consuming due to repetitive protection/deprotection steps. N-Glycans in glycoprotein show structural diversity in its oligosaccharides, the so-called glycoform. Here, I would like to introduce recent synthetic developments focusing on diverse N-linked oligosaccharides based on semisynthesis.

Keywords

Benzyl Ester Pool Fraction Glycosidase Digestion Convenient Synthetic Method Individual Oligosaccharide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Kajihara Y, Suzuki Y, Yamamoto N, Sasaki K, Sakakibara T, Juneja LR (2004) Prompt chemo-enzymatic synthesis of diverse complex-type oligosaccharides and its application to the solid-phase synthesis of a glycopeptide with Asn-linked sialyl-undeca-and asialo-nona-saccharides. Chem Eur J 10(4):971–985CrossRefGoogle Scholar
  2. Seko A, Koketsu M, Nishizono M, Enoki Y, Ibrahim HR, Juneja LR, Kim M, Yamamoto T (1997) Occurrence of a sialylglycopeptide and free sialylglycans in Hen’s egg yolk. Biochim Biophys Acta 1335:23PubMedGoogle Scholar

Copyright information

© Springer 2008

Authors and Affiliations

  • Yasuhiro Kajihara
    • 1
  1. 1.International Graduate School of Arts and SciencesYokohama City UniversityYokohama, KanagawaJapan

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