Construction of O-Linked Glycopeptide Library Using Human Glycosyltransferases

  • Hiromi Ito


Biological functions of glycoconjugates and biomedical applications of carbohydrates have gathered researcher’s attention. The construction of oligosaccharide/glycopeptide libraries would be essential for the development of carbohydrate-based research. We introduce, here, a novel method for the construction of structurally defined glycopeptide library which can be easily converted to an oligosaccharide library using human recombinant glycosyltransferases. In the past few years, approximately 180 glycogenes, which encode glycosyltransferases, sulfotransferases, and sugar-nucleotide transporters, had been cloned. We had created many human glycosyltransferases as a recombinant enzyme, and consequently it is possible to prepare libraries which contain a large variety of oligosaccharide structures using these enzymes.


Recombinant Enzyme Oligosaccharide Structure Glycosyltransferase Gene Single Reaction Tube Human Recombinant Enzyme 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Ito H, Kameyama A, Sato T, Kiyohara K, Nakahara Y, Narimatsu H (2005) Molecular-weight-tagged glycopeptide library: efficient construction and applications. Angew Chem Int Ed 44:4547–4549CrossRefGoogle Scholar
  2. Kameyama A, Kikuchi N, Nakaya S, Ito H, Sato T, Shikanai T, Takahashi Y, Takahashi K, Narimatsu H (2005) A Strategy for identification of oligosaccharide structures using observational multistage mass spectral library. Anal Chem 77:4719–4725PubMedCrossRefGoogle Scholar
  3. Narimatsu H (2004) Construction of a human glycogene library and comprehensive functional analysis. Glycoconj J 21:17–24PubMedCrossRefGoogle Scholar
  4. Sato T, Gotoh M, Kiyohara K, Kameyama A, Kubota T, Kikuchi N, Ishizuka Y, Iwasaki H, Togayachi A, Kudo T, Ohkura T, Nakanishi H, Narimatsu H (2003) Molecular cloning and characterization of a novel human β1,3-glucosyltransferase, β3Glc-T, which is localized at the endoplasmic reticulum and glucosylates O-linked fucosylglycan on thrombospondin type 1 repeat domain. J Biol Chem 278:47534–47544PubMedCrossRefGoogle Scholar
  5. Taniguchi N, Honke K, Fukuda M (eds) (2001) Handbook of glycosyltransferases and related genes. Springer, TokyoGoogle Scholar

Copyright information

© Springer 2008

Authors and Affiliations

  • Hiromi Ito
    • 1
  1. 1.Research Center for Medical GlycoscienceNational Institute of Advanced Industrial Science and Technology (AIST)Tsukuba, IbarakiJapan

Personalised recommendations