Construction of O-Linked Glycopeptide Library Using Human Glycosyltransferases
Biological functions of glycoconjugates and biomedical applications of carbohydrates have gathered researcher’s attention. The construction of oligosaccharide/glycopeptide libraries would be essential for the development of carbohydrate-based research. We introduce, here, a novel method for the construction of structurally defined glycopeptide library which can be easily converted to an oligosaccharide library using human recombinant glycosyltransferases. In the past few years, approximately 180 glycogenes, which encode glycosyltransferases, sulfotransferases, and sugar-nucleotide transporters, had been cloned. We had created many human glycosyltransferases as a recombinant enzyme, and consequently it is possible to prepare libraries which contain a large variety of oligosaccharide structures using these enzymes.
KeywordsRecombinant Enzyme Oligosaccharide Structure Glycosyltransferase Gene Single Reaction Tube Human Recombinant Enzyme
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