Development of Neoglycoconjugate Probes and Detection of Lectins
Glycoconjugates are involved in essential biological processes, and interference with glycosylation is pathological and sometimes lethal in many organisms. Because the biological functions of glycans are exhibited primarily in their interactions with carbohydrate-binding proteins (lectins or receptors), which recognize individual glycoconjugates, we have developed various types of probes to detect lectins and analyze glycan interactions with high sensitivity. Commercially available glycoprobes are prepared by organic synthesis, a method that is suitable for large-scale production but requires large amounts of glycans and specialized synthesis steps. However, preparation of probes by glycobiologists in the laboratory needs to be technically feasible and applicable to only minute amounts of glycans or glycoconjugates, which can be purified from biological sources to provide a probe of adequate sensitivity for detection and to prepare affinity adsorbents. Our probes are prepared by derivatizing natural glycans or glycoconjugates by nondenaturing aqueous-phase reactions. In this report, we describe the development of neoglycoconjugate systems for sensitive lectin screening and interaction analyses. Examples of probes for lectin screening from animal, plant and fungal sources are shown (Ueda et al. 1999; Matsumoto et al. 2001; Ogawa et al. 2003; Satoh et al. 2004).
KeywordsMushroom Extract Fungal Source Biological Ligand Essential Biological Process Natural Glycan
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