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Frontal Affinity Chromatography: An Effective Analytical Tool for Protein-Sugar Interaction

  • Kenichi Kasai

Abstract

Frontal affinity chromatography (FAC) is currently the most powerful tool for studying molecular recognition performed by sugar-binding proteins, and actually the only method that enables detailed profiling of the specificity of sugar-binding proteins, because it has the following distinct merits that no other analytical procedure for molecular interaction has. First, FAC is suitable for the analysis of weak-binding phenomena characteristic to protein-sugar interactions from its principle, although almost all presently available procedures are applicable only to strongly interacting systems. Second, from practical viewpoints, consumption of rare oligosaccharides in FAC can be kept minimal because of its extremely high sensitivity (an amount of ∼20 pmol oligosaccharide is sufficient for one measurement). Even the procedure having the highest sensitivity other than FAC probably requires ten times more sugar molecules. This is why FAC is actually the only available procedure for profiling sugar-binding proteins.

Keywords

Elution Volume Analyte Molecule Practical Viewpoint Elution Curve Small Column 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer 2008

Authors and Affiliations

  • Kenichi Kasai
    • 1
  1. 1.Biochemistry, Faculty of Pharmaceutical SciencesTeikyo UniversitySagamiko, KanagawaJapan

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