Frontal Affinity Chromatography: An Effective Analytical Tool for Protein-Sugar Interaction
Frontal affinity chromatography (FAC) is currently the most powerful tool for studying molecular recognition performed by sugar-binding proteins, and actually the only method that enables detailed profiling of the specificity of sugar-binding proteins, because it has the following distinct merits that no other analytical procedure for molecular interaction has. First, FAC is suitable for the analysis of weak-binding phenomena characteristic to protein-sugar interactions from its principle, although almost all presently available procedures are applicable only to strongly interacting systems. Second, from practical viewpoints, consumption of rare oligosaccharides in FAC can be kept minimal because of its extremely high sensitivity (an amount of ∼20 pmol oligosaccharide is sufficient for one measurement). Even the procedure having the highest sensitivity other than FAC probably requires ten times more sugar molecules. This is why FAC is actually the only available procedure for profiling sugar-binding proteins.
KeywordsElution Volume Analyte Molecule Practical Viewpoint Elution Curve Small Column
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