Development of MS Method for the Analysis of Sugar Peptide
Structural analysis of glycopeptide, positioning the carbohydrate chain as well as its peptide sequencing and carbohydrate sequencing, is quite important for elucidating a function of the glycoprotein. Structural information of N-linked glycopeptide, in particular the kind of N-glycan attached and its position on the peptide, can be determined partially after the treatment with peptide-N-glycosidase F (PNGase F) under 18O water. The released N-glycan structural information can be identified by MS n analysis using mass spectrometer (MS) such as quadrupol ion trap (QIT) MS. The traces of the N-glycan attachments on the PNGase F digested peptide can be detected by MS/MS amino acid sequencing, by which the mass of the N-glycan attached amino acid shifts +2 Da. However, when multiple different N-glycans are attached to a peptide, there is no way to identify the attached position of each N-glycan.
KeywordsLithium Niobate Periodically Pole Lithium Niobate Lithium Tantalate Attached Position Wavelength Tunable Range
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