Construction of a Diagnostic Library for Glycans Using Multistage Tandem Mass Spectrometry (MSn)
Glycomics has lagged far behind proteomics because of difficulties arising from the structural complexity of glycans, such as variations in branching, linkage, and stereochemistry. Recently, tandem mass spectrometry has revealed that different glycan structures have characteristic fragment patterns in their collision-induced dissociation (CID) spectra. We used spectra obtained from a glycan library to construct a multistage tandem mass spectral library (MS n library). This spectral library offers a novel tool for glycomics research, as it enables users to identify glycans very easily and quickly by spectral matching. The MS n library stores triplicate spectra of standard glycans, and information on samples and experimental conditions in a relational database. The strategy of rapid identification is based on comparison of the signal intensity profiles of the spectra obtained by MS n of the analyte with a library of mass spectral data (Kameyama et al. 2005).