Glycan profiling is a novel approach to structural analysis of glycans. Its emphasis is on grasping essential information of target glycans in a rapid, sensitive, and high-throughput manner rather than defining covalent structures in a rigorous and time-consuming manner. This information is particularly important for structural glycomics with the absence of a useful automated sequencer analogous to protein and DNA sequencers, which are based on Edman degradation chemistry and Sanger’s dideoxy method, respectively. However, it is obviously difficult to apply this type of logic to glycans, because they have additional aspects increasing structural variety, which the linear molecules lack. They are represented by linkage isomers (i.e., α/β1–2, 1–3, 1–4, 1–6) and branching events. For this type of branching molecules, it seems that a “memory-matching” or “finger-printing” principle should work better. In this chapter, essence of practical approaches to glycan profiling is described.
KeywordsGlycan Structure Glycan Profile Linkage Isomer Lectin Microarray Intact Live Cell
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