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Glycan Profiling

  • Jun Hirabayashi

Abstract

Glycan profiling is a novel approach to structural analysis of glycans. Its emphasis is on grasping essential information of target glycans in a rapid, sensitive, and high-throughput manner rather than defining covalent structures in a rigorous and time-consuming manner. This information is particularly important for structural glycomics with the absence of a useful automated sequencer analogous to protein and DNA sequencers, which are based on Edman degradation chemistry and Sanger’s dideoxy method, respectively. However, it is obviously difficult to apply this type of logic to glycans, because they have additional aspects increasing structural variety, which the linear molecules lack. They are represented by linkage isomers (i.e., α/β1–2, 1–3, 1–4, 1–6) and branching events. For this type of branching molecules, it seems that a “memory-matching” or “finger-printing” principle should work better. In this chapter, essence of practical approaches to glycan profiling is described.

Keywords

Glycan Structure Glycan Profile Linkage Isomer Lectin Microarray Intact Live Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer 2008

Authors and Affiliations

  • Jun Hirabayashi
    • 1
  1. 1.Lectin Application and Analysis Team, Research Center for Medical GlycoscienceNational Institute of Advanced Industrial Science and Technologym, AISTUmezono, Tsukuba, IbarakiJapan

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