Structural Analyses of Glycoconjugates by NMR
NMR spectroscopy has been one of the most powerful methods to obtain structural information of oligosaccharides. Vliegenthart and co-workers have greatly achieved systematic studies on 1H chemical shifts of oligosaccharides and thereby described numerous structures by using a “structural reporter group” (Vliegenthart 1980). Recent advances in NMR and stable isotope labeling techniques have made possible not only determination of covalent structure of oligosaccharides but also analyses of conformations and dynamics of glycoconjugates, which are usually inaccessible by X-ray crystallography.
KeywordsResidual Dipolar Coupling Stable Isotope Label Spin Coupling Constant Alignment Tensor GlcNAc Residue
Unable to display preview. Download preview PDF.
- Yamaguchi Y, Kato K (2006) Structural glycobiology by stable-isotope-assisted NMR spectroscopy. In: Webb GM (ed) Modern magnetic resonance, vol 1. Springer, The Netherland, pp 219–225Google Scholar