Skip to main content

Structural Analyses of Glycoconjugates by NMR

  • Chapter

Abstract

NMR spectroscopy has been one of the most powerful methods to obtain structural information of oligosaccharides. Vliegenthart and co-workers have greatly achieved systematic studies on 1H chemical shifts of oligosaccharides and thereby described numerous structures by using a “structural reporter group” (Vliegenthart 1980). Recent advances in NMR and stable isotope labeling techniques have made possible not only determination of covalent structure of oligosaccharides but also analyses of conformations and dynamics of glycoconjugates, which are usually inaccessible by X-ray crystallography.

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Duker JM, Serianni AS (1993) (13C)-substituted sucrose: 13C-1H and 13C-13C spin coupling constants to assess furanose ring and glycosidic bond conformations in aqueous solution. Carbohydr Res 249:281–303

    Article  PubMed  CAS  Google Scholar 

  • Prestegard JH, Bougault CM, Kishore AI (2004) Residual dipolar couplings in structure determination of biomolecules. Chem Rev 104:3519–3540

    Article  PubMed  CAS  Google Scholar 

  • Vliegenthart JF (1980) High resolution 1H-NMR spectroscopy of carbohydrate structures. Adv Exp Med Biol 125:77–91

    PubMed  CAS  Google Scholar 

  • Woods RJ, Pathiaseril A, Wormald MR, Edge CJ, Dwek RA (1998) The high degree of internal flexibility observed for an oligomannose oligosaccharide does not alter the overall topology of the molecule. Eur J Biochem 258:372–386

    Article  PubMed  CAS  Google Scholar 

  • Yamaguchi Y, Kato K (2006) Structural glycobiology by stable-isotope-assisted NMR spectroscopy. In: Webb GM (ed) Modern magnetic resonance, vol 1. Springer, The Netherland, pp 219–225

    Google Scholar 

  • Yamaguchi Y, Kim H, Kato K, Masuda K, Shimada I, Arata Y (1995) Proteolytic fragmentation with high specificity of mouse immunoglobulin G. Mapping of proteolytic cleavage sites in the hinge region. J Immunol Methods 181:259–267

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2008 Springer

About this chapter

Cite this chapter

Kato, K., Yamaguchi, Y. (2008). Structural Analyses of Glycoconjugates by NMR. In: Taniguchi, N., Suzuki, A., Ito, Y., Narimatsu, H., Kawasaki, T., Hase, S. (eds) Experimental Glycoscience. Springer, Tokyo. https://doi.org/10.1007/978-4-431-77924-7_13

Download citation

Publish with us

Policies and ethics