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Experimental Glycoscience pp 39–41Cite as

LC/MS of N-Linked Oligosaccharides

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Abstract

Most glycoproteins are attached to some of diverse oligosaccharides differing in their monosaccharide compositions, sequences, branches, linkages, and modifications. Liquid chromatography/mass spectrometry (LC/MS) is recognized as a powerful tool for the separation and characterization of these oligosaccharides (Morelle et al. 2006; Zaia 2004). Here, we present the procedure for the LC/MS of N-linked oligosaccharides released from a glycoprotein.

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References

  • Itoh S, Kawasaki N, Hashii N, Harazono A, Matsuishi Y, Hayakawa T, Kawanishi T (2006) N-linked oligosaccharide analysis of rat brain Thy-1 by liquid chromatography with graphitized carbon column/ion trap-Fourier transform ion cyclotron resonance mass spectrometry in positive and negative ion modes. J Chromatogr A 1103:296–306

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  • Morelle W, Canis K, Chirat F, Faid V, Michalski JC (2006) The use of mass spectrometry for the proteomic analysis of glycosylation. Proteomics 6:3993–4015

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  • Zaia J (2004) Mass spectrometry of oligosaccharides. Mass Spectrom Rev 23:161–227

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Authors and Affiliations

  1. Division of Biological Chemistry and Biologicals, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan

    Nana Kawasaki, Satsuki Itoh & Teruhide Yamaguchi

Authors
  1. Nana Kawasaki
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  2. Satsuki Itoh
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  3. Teruhide Yamaguchi
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Editor information

Editors and Affiliations

  1. Department of Disease Glycomics, Research Institute for Microbial Diseases, Osaka University, 1-1 Yamadaoka, Suita, Osaka, 565-0871, Japan

    Naoyuki Taniguchi M.D., Ph.D. (Professor) (Professor)

  2. Systems Glycobiology Group, Advanced Science Institute, RIKEN, Wako, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan

    Naoyuki Taniguchi M.D., Ph.D. (Professor) (Professor)

  3. Institute of Glycotechnology, Future Science and Technology Joint Research Center, Tokai University, Hiratsuka, Kanagawa, 259-1292, Japan

    Akemi Suzuki M.D., Ph.D. (Professor) (Professor)

  4. RIKEN Discovery Research Institute, Synthetic Cellular Chemistry Laboratory, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan

    Yukishige Ito Ph.D. (Chief Researcher) (Chief Researcher)

  5. Research Center for Medical Glycoscience, Advanced Industrial Science and Technology, AIST Tsukuba, Central 2, Tsukuba, Ibaraki, 305-8568, Japan

    Hisashi Narimatsu M.D., Ph.D.

  6. Research Center for Glycobiotechnology, Ritsumeikan University, 1-1-1 Noji-Higashi, Kusatsu, Shiga, 525-8577, Japan

    Toshisuke Kawasaki Ph.D. (Professor) (Professor)

  7. Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka, 560-0043, Japan

    Sumihiro Hase Ph.D. (Professor) (Professor)

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© 2008 Springer

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Kawasaki, N., Itoh, S., Yamaguchi, T. (2008). LC/MS of N-Linked Oligosaccharides. In: Taniguchi, N., Suzuki, A., Ito, Y., Narimatsu, H., Kawasaki, T., Hase, S. (eds) Experimental Glycoscience. Springer, Tokyo. https://doi.org/10.1007/978-4-431-77924-7_11

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