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LC/MS of N-Linked Oligosaccharides

  • Nana Kawasaki
  • Satsuki Itoh
  • Teruhide Yamaguchi

Abstract

Most glycoproteins are attached to some of diverse oligosaccharides differing in their monosaccharide compositions, sequences, branches, linkages, and modifications. Liquid chromatography/mass spectrometry (LC/MS) is recognized as a powerful tool for the separation and characterization of these oligosaccharides (Morelle et al. 2006; Zaia 2004). Here, we present the procedure for the LC/MS of N-linked oligosaccharides released from a glycoprotein.

Keywords

Follicle Stimulate Hormone Guanidine Hydrochloride Monosaccharide Composition Typical Mass Spectrum Human Follicle Stimulate Hormone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Itoh S, Kawasaki N, Hashii N, Harazono A, Matsuishi Y, Hayakawa T, Kawanishi T (2006) N-linked oligosaccharide analysis of rat brain Thy-1 by liquid chromatography with graphitized carbon column/ion trap-Fourier transform ion cyclotron resonance mass spectrometry in positive and negative ion modes. J Chromatogr A 1103:296–306PubMedCrossRefGoogle Scholar
  2. Morelle W, Canis K, Chirat F, Faid V, Michalski JC (2006) The use of mass spectrometry for the proteomic analysis of glycosylation. Proteomics 6:3993–4015PubMedCrossRefGoogle Scholar
  3. Zaia J (2004) Mass spectrometry of oligosaccharides. Mass Spectrom Rev 23:161–227PubMedCrossRefGoogle Scholar

Copyright information

© Springer 2008

Authors and Affiliations

  • Nana Kawasaki
    • 1
  • Satsuki Itoh
    • 1
  • Teruhide Yamaguchi
    • 1
  1. 1.Division of Biological Chemistry and BiologicalsNational Institute of Health SciencesTokyoJapan

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