Most glycoproteins are attached to some of diverse oligosaccharides differing in their monosaccharide compositions, sequences, branches, linkages, and modifications. Liquid chromatography/mass spectrometry (LC/MS) is recognized as a powerful tool for the separation and characterization of these oligosaccharides (Morelle et al. 2006; Zaia 2004). Here, we present the procedure for the LC/MS of N-linked oligosaccharides released from a glycoprotein.
KeywordsFollicle Stimulate Hormone Guanidine Hydrochloride Monosaccharide Composition Typical Mass Spectrum Human Follicle Stimulate Hormone
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