Chemical Liberation of N-linked Oligosaccharides from Glycoproteins
When N-linked oligosaccharides are chemically liberated from the glycoproteins, the hydrazine anhydrous is generally used. Hydrazinolysis has been originally used as a method to determine carboxyl terminal amino acid of protein. Yoshizawa et al. (1966) found that the method is well applicable to chemical liberation of oligosaccharides from glycoproteins. Upon hydrazinolysis, lyophilized samples including glycoproteins are completely solved in hydrazine, and thus, the reaction proceeds smoothly. After hydrazinolysis, released acetyl group of amino sugars, such as GlcNAc and GalNAc, must be subjected to re-acetylation (Takasaki et al. 1982) (Fig. 1).
KeywordsAcetic Anhydride Amino Sugar Seal Tube Rotary Pump Enzymatic Procedure
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