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KO Mice of α-2,8-sialyltransferase (GD3 Synthase)

  • Koichi Furukawa
  • Masahiko Okada
  • Keiko Furukawa

Abstract

Gangliosides have been considered to play important roles in the development and differentiation of nervous systems in vertebrates. In particular, b-series gangliosides were reported to be effective as a neurotrophic factor, e.g. as inducer of neurite extension. In order to directly address these functions, we generated knockout mice of α-2,8-sialyltransferase gene (Haraguchi et al. 1994) that is responsible for the synthesis of GD3 (and GT3), and clearly showed that this enzyme is really critical for the synthesis of b-series gangliosides (Okada et al. 2002). The null mutant mice showed no apparent neurological disorders, and their brain and nervous tissues were almost normally generated. They showed no defects in spermatogenesis and immunological examination. However, the null mutants demonstrated reduced neuroregeneration in the hypoglossal resection experiments, suggesting that b-series gangliosides are important in the repair of lesioned nerves (Okada et al. 2002).

Keywords

Hypoglossal Nerve Null Mutant Mouse Immunological Examination Sialyltransferase Gene 
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References

  1. Handa Y, Ozaki N, Honda T, Furukawa K, Tomita Y, Inoue M, Okada M, Furukawa K, Sugiura Y (2005) GD3 synthase gene knockout mice exhibit thermal hyperalgesia and mechanical allodynia but decreased response to formalin induced prolonged noxious stimulation. Pain 117:271–279PubMedCrossRefGoogle Scholar
  2. Haraguchi M, Yamashiro S, Yamamoto A, Furukawa K, Takamiya K, Lloyd KO, Shiku H, Furukawa K (1994) Isolation of GD3 synthase gene by expression cloning of GM3 alpha-2,8-sialyltransferase cDNA using anti-GD2 monoclonal antibody. Proc Natl Acad Sci USA 91:10455–10459PubMedCrossRefGoogle Scholar
  3. Okada M, Itoh M, Haraguchi M, Okajima T, Inoue M, Ohishi H, Matsuda Y, Iwamoto T, Kawano T, Fukumoto S, Miyazaki H, Furukawa K, Aizawa S, Furukawa K (2002) b-Series ganglioside deficiency exhibits no definite changes in the neurogenesis and the sensitivity to Fas-mediated apoptosis, but impairs regeneration of the lesioned hypoglossal nerve. J Biol Chem 277:1633–1636PubMedCrossRefGoogle Scholar

Copyright information

© Springer 2008

Authors and Affiliations

  • Koichi Furukawa
    • 1
  • Masahiko Okada
    • 1
  • Keiko Furukawa
    • 1
  1. 1.Department of Biochemistry IINagoya University Graduate School of MedicineNagoyaJapan

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