KO Mice of Cerebroside Sulfotransferase
Sulfatide synthase catalyzes the transfer of sulfonate group to the C3 position of the nonreducing terminal galactose of glycolipid oligosaccharides, and is traditionally called cerebroside sulfotransferase (CST). We purified CST homogeneously from human renal cancer cells (Honke et al. 1996) and subsequently isolated a cDNA clone of CST from a cDNA library of human renal cancer cells on the basis of the partial amino acid sequences of the purified enzyme (Honke et al. 1997). Furthermore, we cloned mouse cDNA and genomic DNA (Hirahara et al. 2000). In order to know the biological roles of the sulfation of glycolipids and to determine whether a single enzyme is responsible for the biosynthesis of sulfatide and seminolipid which are different in their lipid moiety, CST-deficient mice were created by gene targeting (Honke et al. 2002).
KeywordsSulfonate Group Ureteral Obstruction Lipid Moiety Partial Amino Acid Sequence Progressive Ataxia
- Ogawa D, Shikata K, Honke K, Sato S, Matsuda M, Nagase R, Tone A, Okada S, Usui H, Wada J, Miyasaka M, Kawashima H, Suzuki Y, Suzuki T, Taniguchi N, Hirahara Y, Tadano-Aritomi K, Ishizuka I, Tedder TF, Makino H (2004) Cerebroside sulfotransferase deficiency ameliorates lselectin-dependent monocyte infiltration in the kidney after ureteral obstruction. J Biol Chem 279:2085–2090PubMedCrossRefGoogle Scholar