KO Mice of β1,4-galactosyltransferase-I
β1,4-galactosyltransferases (β4GalTs) transfer galactose (Gal) from UDP-Gal to terminal GlcNAc of N- and O-glycans in a β1,4-linkage to synthesize the Galβ1-4GlcNAc structure. So far, seven β4GalT genes (β4GalT-I to VII) have been isolated and six of them are shown to be involved in the biosynthesis of the Galβ1-4GlcNAc structure. β4GalT-I is expressed ubiquitously and strongly in almost all tissues except neural tissues, suggesting that β4GalT-I is engaged in β4-galactosylation of many glycoproteins. β4-galactosylation of glycoproteins is widely distributed in mammalian tissues and involved in various physiological functions through interactions with selectins, galectins, asialoglycoprotein receptor (ASGPR) and so on. We, as well as Shur’s group, generated mice lacking in β4GalT-I to elucidate the role of β4-galactosylation in vivo (Asano et al. 1997; Lu et al. 1997).
KeywordsSkin Wound Healing Aggregation Plate Terminal GlcNAc Chimera Mouse Negative Selection Marker
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