KO Mice of β1,4-galactosyltransferase-I

  • Masahide Asano


β1,4-galactosyltransferases (β4GalTs) transfer galactose (Gal) from UDP-Gal to terminal GlcNAc of N- and O-glycans in a β1,4-linkage to synthesize the Galβ1-4GlcNAc structure. So far, seven β4GalT genes (β4GalT-I to VII) have been isolated and six of them are shown to be involved in the biosynthesis of the Galβ1-4GlcNAc structure. β4GalT-I is expressed ubiquitously and strongly in almost all tissues except neural tissues, suggesting that β4GalT-I is engaged in β4-galactosylation of many glycoproteins. β4-galactosylation of glycoproteins is widely distributed in mammalian tissues and involved in various physiological functions through interactions with selectins, galectins, asialoglycoprotein receptor (ASGPR) and so on. We, as well as Shur’s group, generated mice lacking in β4GalT-I to elucidate the role of β4-galactosylation in vivo (Asano et al. 1997; Lu et al. 1997).


Skin Wound Healing Aggregation Plate Terminal GlcNAc Chimera Mouse Negative Selection Marker 
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  1. Asano M, Furukawa K, Kido M, Matsumoto S, Umesaki Y, Kochibe N, Iwakura Y (1997) Growth retardation and early death of β-1,4-galactosyltransferase knockout mice with augmented proliferation and abnormal differentiation of epithelial cells. EMBO J 16:1850–1857PubMedCrossRefGoogle Scholar
  2. Asano M, Nakae S, Kotani N, Shirafuji N, Nambu A, Hashimoto N, Kawashima H, Hirose M, Miyasaka M, Takasaki S, Iwakura Y (2003) Impaired selectin-ligand biosynthesis and reduced inflammatory responses in β-1,4-galactosyltransferase-I-deficient mice. Blood 102:1678–1685PubMedCrossRefGoogle Scholar
  3. Kotani N, Asano M, Iwakura Y and Takasaki S (2001) Knockout of mouse β1,4-galactosyltransferase-1 gene results in a dramatic shift of outer chain moieties of N-glycans from type 2 to type 1 chains in hepatic membrane and plasma glycoproteins. Biochem J 357:827–834PubMedCrossRefGoogle Scholar
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  5. Mori R, Kondo T, Nishie T, Ohshima T, Asano M. (2004) Impairment of skin wound healing in β-1, 4-galactosyltransferase-deficient mice with reduced leukocyte recruitment. Am J Pathol 164:1303–1314PubMedGoogle Scholar

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© Springer 2008

Authors and Affiliations

  • Masahide Asano
    • 1
  1. 1.Advanced Science Research CenterKanazawa UniversityKanazawaJapan

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