Studies on Functions of Notch O-Fucosylation in Drosophila
Notch signaling is an evolutionarily conserved mechanism that regulates a broad spectrum of cell-specification events through local cell-cell communication. Notch encodes a single-pass transmembrane receptor protein with 36 epidermal growth factor-like (EGF) repeats. The EGF-like repeats of the Notch extracellular domain, which contain a consensus sequence, are modified by the O-linked tetrasaccharide Sia-α2,3-Gal-β1,4-GlcNAc-β1,3-Fuc (Fig. 1A). A GDP-fucose protein O-fucosyltransferase1 catalyzes this O-linked fucosylation (Wang et al. 2001). In Drosophila, this enzyme is encoded by O-fut1.
KeywordsNotch Signaling Mutant Embryo Wing Disc Aleuria Aurantia Lectin Salt Monohydrate
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