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O-Mannosyltransferase and POMGnT1

  • Tamao Endo
  • Hiroshi Manya

Abstract

O-Mannosylation is important in muscle and brain development. We previously found that the glycans of α-dystroglycan (α-DG) predominantly include O-mannosyl glycans, Siaα2-3Galβ1-4GlcNAcβ1-2Man (Chiba et al. 1997), and then we reported that defects in O-mannosyl glycan cause a type of muscular dystrophy. We have identified and characterized glycosyltransferases; protein O-mannose β1,2-N-acetylglucosaminyltransferase (POMGnT1) (Yoshida et al. 2001) and protein O-mannosyltransferase 1 (POMT1) and its homolog POMT2 (Manya et al. 2004) are involved in O-mannosyl glycan synthesis. This protocol describes assay methods for the mammalian POMT and POMGnT1.

Keywords

Muscular Dystrophy Tokyo Metropolitan Institute Rotary Mixer Human Embryonic Kidney 293T Acterized Glycosyltransferases 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Chiba A, Matsumura K, Yamada H, Inazu T, Shimizu T, Kusunoki S, Kanazawa I, Kobata A, Endo T (1997) Structures of sialylated O-linked oligosaccharides of bovine peripheral nerve α-dystroglycan: the role of a novel O-mannosyl-type oligosaccharide in the binding of α-dystroglycan with laminin. J Biol Chem 272:2156–2162PubMedCrossRefGoogle Scholar
  2. Manya H, Chiba A, Yoshida A, Wang X, Chiba Y, Jigami Y, Margolis RU, Endo T (2004) Demonstration of mammalian protein O-mannosyltransferase activity: coexpression of POMT1 and POMT2 required for enzymatic activity. Proc Natl Acad Sci USA 101:500–505PubMedCrossRefGoogle Scholar
  3. Yoshida A, Kobayashi K, Manya H, Taniguchi K, Kano H, Mizuno M, Inazu T, Mitsuhashi H, Takahashi S, Takeuchi M, Herrmann R, Straub V, Talim B, Voit T, Topaloglu H, Toda T, Endo T (2001) Muscular dystrophy and neuronal migration disorder caused by mutations in a glycosyltransferase, POMGnT1. Dev Cell 1:717–724PubMedCrossRefGoogle Scholar

Copyright information

© Springer 2008

Authors and Affiliations

  • Tamao Endo
    • 1
  • Hiroshi Manya
    • 1
  1. 1.Glycobiology Research Group, Tokyo Metropolitan Institute of GerontologyFoundation for Research on Aging and Promotion of Human WelfareTokyoJapan

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