O-Mannosyltransferase and POMGnT1
O-Mannosylation is important in muscle and brain development. We previously found that the glycans of α-dystroglycan (α-DG) predominantly include O-mannosyl glycans, Siaα2-3Galβ1-4GlcNAcβ1-2Man (Chiba et al. 1997), and then we reported that defects in O-mannosyl glycan cause a type of muscular dystrophy. We have identified and characterized glycosyltransferases; protein O-mannose β1,2-N-acetylglucosaminyltransferase (POMGnT1) (Yoshida et al. 2001) and protein O-mannosyltransferase 1 (POMT1) and its homolog POMT2 (Manya et al. 2004) are involved in O-mannosyl glycan synthesis. This protocol describes assay methods for the mammalian POMT and POMGnT1.
KeywordsMuscular Dystrophy Tokyo Metropolitan Institute Rotary Mixer Human Embryonic Kidney 293T Acterized Glycosyltransferases
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