Functional Glycomics at the Level of Single Cells: Studying Roles of Sugars in Cell Division, Differentiation, and Morphogenesis with 4D Microscopy
The nematode Caenorhabditis elegans is the first multicellular organism whose complete cell lineage, cellular anatomy, and neural wiring diagram of its nervous system are determined. Because the complete DNA sequence of its genome is also available, it is easy to isolate deletion mutants from TMP (trimethylpsoralen)/UV mutagenized library of animals by PCR screening with carefully designed primer sets (cf. http://shigen.lab.nig.ac.jp/c.elegans/ChangeLocale.do?lang=en&url=home). Mutant strains can be stored frozen indefinitely, and this makes this model organism the only multicellular organism compatible with high throughput biologic study. In addition, genes defined by sequence similarity to any known genes can be easily identified and mutated, and the phenotypes of mutant animals can be studied at the level of single cells as the body of the nematode is transparent.
KeywordsHard Disk Drive Nematode Caenorhabditis Elegans Early Embryonic Cell White Petrolatum Hard Disk Space
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