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Functional Analysis of Sugar Chains Using a Genome-Wide RNAi System in Drosophila

  • Shoko Nishihara

Abstract

Drosophila melanogaster was first used for genetic studies by Thomas Hunt Morgan in 1908. Drosophila has since become one of the most important model organisms for developmental biology studies in addition to those of classical and molecular genetics. Recently, effective analysis of glycan functions in vivo has been achieved using Drosophila. Flies with mutations of some glycosyltransferases display distinctive phenotypes, and these mutant flies have been invaluable in clarifying the role of glycosylation in Wg/Wnt, Hh, FGF, and Notch signaling in vivo. These data emphasize the crucial importance of protein glycosylation for many biological processes and indicate that glycobiology will provide a new focus of investigation for many aspects of Biology and Medicine.

Keywords

Sugar Chain RNAi System Guide Strand GAL4 Driver Target Gene Sequence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Goda E, Kamiyama S, Uno T, Yoshida H, Ueyama M, Kinoshita-Toyoda A, Toyoda H, Ueda R, Nishihara S (2006) Identification and characterization of a novel Drosophila 3′-phosphoadenosine 5′-phosphosulfate transporter. J Biol Chem 281:28508–28517PubMedCrossRefGoogle Scholar
  2. Ichimiya T, Manya H, Ohmae Y, Yoshida H, Takahashi K, Ueda R, Endo T, Nishihara S (2004) The twisted abdomen phenotype of Drosophila POMT1 and POMT2 mutants coincides with their heterophilic protein O-mannosyltransferase activity. J Biol Chem 279:42638–42647PubMedCrossRefGoogle Scholar
  3. Kamiyama S, Suda T, Ueda R, Suzuki M, Okubo R, Kikuchi N, Chiba Y, Goto S, Toyoda H, Saigo K, Watanabe M, Narimatsu H, Jigami Y, Nishihara S (2003) Molecular cloning and identification of 3′-phosphoadenosine 5′-phosphosulfate transporter. J Biol Chem 278:25958–25963PubMedCrossRefGoogle Scholar
  4. Nishihara S, Ueda R, Goto S, Toyoda H, Ishida H, Nakamura M (2004) Approach for functional analysis of glycan using RNA interference. Glycoconj J 21:63–68PubMedCrossRefGoogle Scholar
  5. Takemae H, Ueda R, Okubo R, Nakato H, Izumi S, Saigo K, Nishihara S (2003) Proteoglycan UDP-galactose: beta-xylose β1,4-galactosyltransferase I is essential for viability in Drosophila melanogaster. J Biol Chem 278:15571–15578PubMedCrossRefGoogle Scholar

Copyright information

© Springer 2008

Authors and Affiliations

  • Shoko Nishihara
    • 1
  1. 1.Laboratory of Cell Biology, Department of Bioinformatics, Faculty of EngineeringSoka UniversityHachioji, TokyoJapan

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