Functional Analysis of Sugar Chains Using a Genome-Wide RNAi System in Drosophila
Drosophila melanogaster was first used for genetic studies by Thomas Hunt Morgan in 1908. Drosophila has since become one of the most important model organisms for developmental biology studies in addition to those of classical and molecular genetics. Recently, effective analysis of glycan functions in vivo has been achieved using Drosophila. Flies with mutations of some glycosyltransferases display distinctive phenotypes, and these mutant flies have been invaluable in clarifying the role of glycosylation in Wg/Wnt, Hh, FGF, and Notch signaling in vivo. These data emphasize the crucial importance of protein glycosylation for many biological processes and indicate that glycobiology will provide a new focus of investigation for many aspects of Biology and Medicine.
KeywordsSugar Chain RNAi System Guide Strand GAL4 Driver Target Gene Sequence
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