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A Cytoplasmic Peptide: N-Glycanase and ER-Associated Degradation

  • Tadashi Suzuki

Abstract

Peptide: N-glycanase (PNGase) hydrolyzes the amide bond between proximal GlcNAc and the linkage Asn residue of N-linked glycopeptides/glycoproteins, releasing free oligosaccharides bearing N,N′-diacetylchitobiose structure at their reducing termini (after spontaneous release of ammonia). This enzyme has been widely used as a tool reagent for analyzing the structure and functions of N-linked glycan chains on glycoproteins. The occurrence of cytoplasmic PNGase activity has been reported in a wide variety of eukaryotes (Suzuki et al. 2002 and references therein). The cytoplasmic PNGase was found to be quite distinct in terms of enzymatic properties from the “reagent” PNGases of plant and bacterial origin. A gene encoding the cytoplasmic enzyme, PNG1, was first identified in Saccharomyces cerevisiae (Suzuki et al. 2000). This gene has highly conserved its orthologues and can be found throughout eukaryotes (Suzuki et al. 2002).

Keywords

Paper Electrophoresis ERAD Substrate Bovine Pancreatic Ribonuclease Free Oligosaccharide Glass Bead Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Seko A, Kitajima K, Inoue Y, Inoue S (1991) Peptide:N-glycosidase activity found in the early embryos of Oryzias latipes (Medaka fish): the first demonstration of the occurrence of peptide:N-glycosidase in animal cells and its implication for the presence of a de-N-glycosylation system in living organisms. J Biol Chem 266:22110–22114PubMedGoogle Scholar
  2. Suzuki T (2005) A simple, sensitive in vitro assay for cytoplasmic deglycosylation by peptide: N-glycanase. Methods 35:360–365PubMedCrossRefGoogle Scholar
  3. Suzuki T, Park H, Hollingsworth NM, Sternglanz R, Lennarz WJ (2000) PNG1, a yeast gene encoding a highly conserved peptide:N-glycanase. J Cell Biol 149:1039–1052PubMedCrossRefGoogle Scholar
  4. Suzuki T, Park H, Lennarz WJ (2002) Cytoplasmic peptide:N-glycanase (PNGase) in eukaryotic cells: occurrence, primary structure, and potential functions. FASEB J 16:635–641PubMedCrossRefGoogle Scholar
  5. Tanabe K, Lennarz WJ, Suzuki T (2006) A cytoplasmic peptide:N-glycanase. Methods Enzymol 415:46–55PubMedCrossRefGoogle Scholar

Copyright information

© Springer 2008

Authors and Affiliations

  • Tadashi Suzuki
    • 1
  1. 1.Glycometabolome Laboratory, Systems Glycobiology Research Group, Frontier Research SystemRIKEN InstituteWako, SaitamaJapan

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