Affinity Purification of Recombinant Galectins
Galectins are multifunctional animal lectins defined as β-galactoside-binding proteins with conserved carbohydrate-recognition domains (CRDs). They are largely classified into three structural types: proto, chimera, and tandem-repeat types (Kasai and Hirabayashi 1996). The proto- and chimera-type galectins are composed of a single CRD and form noncovalent multimers, typically dimer of prototype ones. On the other hand, the tandemrepeat type galectins consist of two distinct CRDs connected by a linker polypeptide. Most galectins exhibit hemagglutinin activity inhibited by β-galactosides, such as lactose.
KeywordsLuria Bertani Xylanase Activity Galectin Expression Molecular Mass Marker Amicon Ultra
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