Heparan Sulfate Endosulfatase Assay

  • Kazuko Keino-Masu
  • Masayuki Masu


Heparan sulfate (HS) shows enormous structural heterogeneity generated by the combined modification of epimerization, N-sulfation, and O-sulfation in the polysaccharide chain. A novel family of extracellular sulfatases (SulfFP1/sulf-1 and SulfFP2/sulf-2) identified in quail, rats, humans, and mice (Dhoot et al. 2001; Morimoto-Tomita 2002; Ohto et al. 2002) act as endosulfatases, which remove 6-O sulfate from N-acetylglucosamine residues in intact heparin/HS. Previous studies have revealed that these endosulfatases modulated the signaling of many heparin-binding factors, suggesting that they play a role in the regulation of cellular signaling in vivo. Here, we describe a method to measure the endosulfatase activity.


Conditioned Medium Heparan Sulfate Choroid Plexus Hydrogen Sulfate Disaccharide Unit 
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Copyright information

© Springer 2008

Authors and Affiliations

  • Kazuko Keino-Masu
    • 1
  • Masayuki Masu
    • 1
  1. 1.Department of Molecular Neurobiology, Institute of Basic Medical Sciences, Graduate School of Comprehensive Human SciencesUniversity of TsukubaTsukuba, IbarakiJapan

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