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Heparan Sulfate Endosulfatase Assay

  • Kazuko Keino-Masu
  • Masayuki Masu

Abstract

Heparan sulfate (HS) shows enormous structural heterogeneity generated by the combined modification of epimerization, N-sulfation, and O-sulfation in the polysaccharide chain. A novel family of extracellular sulfatases (SulfFP1/sulf-1 and SulfFP2/sulf-2) identified in quail, rats, humans, and mice (Dhoot et al. 2001; Morimoto-Tomita 2002; Ohto et al. 2002) act as endosulfatases, which remove 6-O sulfate from N-acetylglucosamine residues in intact heparin/HS. Previous studies have revealed that these endosulfatases modulated the signaling of many heparin-binding factors, suggesting that they play a role in the regulation of cellular signaling in vivo. Here, we describe a method to measure the endosulfatase activity.

Keywords

Conditioned Medium Heparan Sulfate Choroid Plexus Hydrogen Sulfate Disaccharide Unit 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Springer 2008

Authors and Affiliations

  • Kazuko Keino-Masu
    • 1
  • Masayuki Masu
    • 1
  1. 1.Department of Molecular Neurobiology, Institute of Basic Medical Sciences, Graduate School of Comprehensive Human SciencesUniversity of TsukubaTsukuba, IbarakiJapan

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