Release of Sugar Chains from Glycosphingolipids
Sugar chains of glycosphingolipids (GSLs) can be released by ozonolysis or periodate oxidation; however, these methods are not suitable for alkaline-sensitive GSLs because they are performed under alkaline conditions. Furthermore, these chemical procedures are time-consuming, somewhat troublesome, and of low yield; and thus a simple and reproducible method for obtaining intact sugar chains from GSLs is required. This section deals with the release of sugar chains from GSLs by endoglycoceramidase (EGCase, EC188.8.131.52).
KeywordsSodium Acetate Buffer Recombinant Form Periodate Oxidation Sugar Chain Ceramide Moiety
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