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α1,4-Linkage Glycosyltransferases

  • Jun Nakayama
  • Koichi Furukawa

Abstract

Glycosyltransferases that transfer GlcNAc and Gal to βGal residues with α1,4-linkages on glycoprotein and glycolipid templates, respectively, are categorized as α1,4-linkage glycosyltransferases. At present, two enzymes, namely, α1,4-N-acetylglucosaminyltransferase (α4GnT) and α1,4-galactosyltransferase (α4GalT, Gb3/CD77 synthase) are assigned to this family. α4GnT is responsible for biosynthesis of GlcNAcα1 → 4Galβ → R attached to O-glycans, whereas α4GalT is a key enzyme in the formation of Gb3/CD77 (Fig. 1). Since cDNAs encoding both enzymes were cloned by expression cloning by the authors (Nakayama et al. 1999; Kojima et al. 2000), we describe here the procedure for expression cloning of α1,4-linkage glycosyltransferases, focusing primarily on α4GnT.

Keywords

Expression Cloning Megakaryoblastic Leukemia Gland Mucous Cell Sibling Selection Infected Mouse Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer 2008

Authors and Affiliations

  • Jun Nakayama
    • 1
  • Koichi Furukawa
    • 2
  1. 1.Department of PathologyShinshu University School of MedicineMatsumotoJapan
  2. 2.Department of Biochemistry IINagoya University School of MedicineNagoyaJapan

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