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Glucuronyltransferases Involved in the HNK-1 Biosynthesis

  • Shinako Kakuda
  • Toshisuke Kawasaki
  • Shogo Oka

Abstract

The monoclonal antibody HNK-1 was raised against the membrane fraction of the human HSB-2 T-cell line. The antigen was originally found to be a marker of human natural killer (HNK) cells and is called as CD57 in immunology. After that, it was found that the HNK-1 carbohydrate epitope is highly expressed in the nervous system, especially on a series of cell adhesion molecules, including neural cell adhesion molecule (NCAM), myelin-associated glycoprotein (MAG), L1, P0, telencephalin, and also some glycolipids. The structure of the HNK-1 epitope was demonstrated to comprise the sulfated trisaccharide HSO3-3GlcAβ1-3Galβ1-4GlcNAc, which is shared by glycolipids and glycoproteins. The expression of the HNK-1 carbohydrate epitope is spatially and temporally regulated during the development of the nervous system, and it functions in cell adhesion, migration, neurite outgrowth and synaptic plasticity (Yamamoto et al. 2002).

Keywords

Synaptic Plasticity Neurite Outgrowth Neural Cell Adhesion Molecule Acceptor Substrate Partial Amino Acid Sequence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Kakuda S, Shiba T, Ishiguro M, Tagawa H, Oka S, Kajihara Y, Kawasaki T, Wakatsuki S, Kato R (2004) Structural basis for acceptor substrate recognition of a human glucuronyltransferase, GlcAT-P, an enzyme critical in the biosynthesis of the carbohydrate epitope HNK-1. J Biol Chem 279: 22693–22703PubMedCrossRefGoogle Scholar
  2. Kakuda S, Sato Y, Tonoyama Y, Oka S, Kawasaki T (2005) Different acceptor specificities of two glucuronyltransferases involved in the biosynthesis of HNK-1 carbohydrate. Glycobiology 15:203–210PubMedCrossRefGoogle Scholar
  3. Shiba T, Kakuda S, Ishiguro M, Morita I, Oka S, Kawasaki T, Wakatsuki S, Kato R (2006) Proteins 65:499–508PubMedCrossRefGoogle Scholar
  4. Yamamoto S, Oka S, Inoue M, Shimuta M, Manabe T, Takahashi H, Miyamoto M, Asano M, Sakagami J, Sudo K, Iwakura Y, Ono K, Kawasaki T (2002) Mice deficient in nervous system-specific carbohydrate epitope HNK-1 exhibit impaired synaptic plasticity and spatial learning. J Biol Chem 277:27227–27231PubMedCrossRefGoogle Scholar

Copyright information

© Springer 2008

Authors and Affiliations

  • Shinako Kakuda
    • 1
  • Toshisuke Kawasaki
    • 2
  • Shogo Oka
    • 1
  1. 1.Department of Biological Chemistry, School of Health Sciences, Faculty of MedicineKyoto UniversityKyotoJapan
  2. 2.Research Center for GlycobiotechnologyRitsumeikan UniversityShigaJapan

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