Glucuronyltransferases Involved in the HNK-1 Biosynthesis
The monoclonal antibody HNK-1 was raised against the membrane fraction of the human HSB-2 T-cell line. The antigen was originally found to be a marker of human natural killer (HNK) cells and is called as CD57 in immunology. After that, it was found that the HNK-1 carbohydrate epitope is highly expressed in the nervous system, especially on a series of cell adhesion molecules, including neural cell adhesion molecule (NCAM), myelin-associated glycoprotein (MAG), L1, P0, telencephalin, and also some glycolipids. The structure of the HNK-1 epitope was demonstrated to comprise the sulfated trisaccharide HSO3-3GlcAβ1-3Galβ1-4GlcNAc, which is shared by glycolipids and glycoproteins. The expression of the HNK-1 carbohydrate epitope is spatially and temporally regulated during the development of the nervous system, and it functions in cell adhesion, migration, neurite outgrowth and synaptic plasticity (Yamamoto et al. 2002).
KeywordsSynaptic Plasticity Neurite Outgrowth Neural Cell Adhesion Molecule Acceptor Substrate Partial Amino Acid Sequence
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