Carbohydrate Microarray for Deciphering the Information Embedded in Oligosaccharide Structures

  • Shigeyuki Fukui


The neoglycolipid (NGL) technology for generating lipid-linked oligosaccharide probes has been shown to be an extremely useful tool for detection and characterization of bioactive oligosaccharides that are extremely minor components (Feizi and Child 1994). As the affinities of most carbohydrate-protein interactions are thought to be very low, and the oligosaccharide portions that play a role in biological function are generally believed to be of a small region ranging from monosaccharide to decasaccharide, the neoglycolipid technology is eminently adaptable for microarray design for high-throughput detection and specificity assignments of carbohydrate-protein interactions.


Hepatocyte Growth Factor Keratinocyte Growth Factor Microarray Design HPTLC Plate Iduronic Acid 


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  1. Feizi T, Child RA (1994) Neoglycolipids: probes in structure/function assignments to oligosaccharides. Methods Enzymol 242:205–217PubMedCrossRefGoogle Scholar
  2. Feizi T et al (1994) Methods Enzymol 230:484–519PubMedCrossRefGoogle Scholar
  3. Fukui S, Feizi T, Galustian C, Lawson AM, Chai W (2002) Oligosaccharide microarrays for high-throughput detection and specificity assignments of carbohydrate-protein interactions. Nat Biotechnol 20:1011–1017PubMedCrossRefGoogle Scholar
  4. Houseman BT, Mrksich M (2002) Model systems for studying polyvalent carbohydrate binding interactions. Top Curr Chem 218:1–44CrossRefGoogle Scholar
  5. Schwarz M, Spector L, Gargir A, Shtevi A, Gortler M, Altstock RT, Dukler A, Dotan N (2003) A new kind of carbohydrate array, its use for profiling antiglycan antibodies, and the discovery of a novel human cellulose-binding antibody. Glycobiology 13:749–754PubMedCrossRefGoogle Scholar
  6. Yamaguchi K, Tamaki T, Fukui S (2006) Detection of oligosaccharide ligands for Hepatocyte growth factor/Scatter factor (HGF/SF), Keratinocyte growth factor (KGF/FGF-7), RANTES and Heparin cofactor II by neoglycolipid microarrays of glycosaminoglycan-derived oligosaccharide fragments. Glycoconjugate J 23(7–8):513–523CrossRefGoogle Scholar

Copyright information

© Springer 2008

Authors and Affiliations

  • Shigeyuki Fukui
    • 1
  1. 1.Department of Biotechnology, Faculty of EngineeringKyoto Sangyo UniversityKyotoJapan

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