Lectin-based glycan profiling is an emerging technique to estimate an entire feature of glycosylation of glycoproteins and cells through biochemical interaction analysis between the analyte glycan and multiple lectins. Hereby, the strategy is basically distinct from other conventional methods represented by MS and HPLC (Hirabayashi 2004). Lectin microarray described in this chapter is one of the most sensitive, rapid and high-throughput profiling methods, which enables analysis of over a 100 of lectin-glycan interactions in a simultaneous manner. However, the biggest charm of the lectin microarray is its simple manipulation in order to analyze glycan structures in both pure and crude forms of glycoproteins, because analyte glycans need not be released from protein moiety nor highly purified as in the cases of HPLC and MS analyses. In fact, the lectin microarray has been recognized as a unique method to analyze glycosylation feature of diverse glycoproteins (Kuno et al. 2005; Rosenfeld et al. 2007), which include those of crude cell lysates, sera, and bacteria (Zheng et al. 2005; Ebe et al. 2006; Hsu et al. 2006; Pilobello et al. 2007). Through comparative microarray analysis (i.e., differential profiling), structural differences can be detected as the changes in signal patterns of the lectin-binding intensities. Therefore, this system is a good means for quality control of various glycoprotein products (e.g., antibody drugs), differential analysis of appropriate clinical samples for investigating useful glyco-biomarkers, and so on.
KeywordsCrude Cell Lysate High Background Noise Operation Software Simultaneous Manner Lectin Microarray
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