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GALAXY Database and Pyridylaminated Oligosaccharide Library

  • Koichi Kato
  • Noriko Takahashi

Abstract

A wide array of standard oligosaccharides is a valuable glycomics tool for systematic and detailed analyses of structures and functions of sugar chains. The multi-dimensional HPLC mapping method developed by Takahashi and co-workers enables us to isolate N-linked oligosaccharides as pyridylaminated (PA) derivatives, discriminating isomeric structures. Therefore it becomes extremely useful not only for identification and profiling of N-glycans but also for purification and library formation of PA-oligosaccharides (Tomiya et al. 1998; Takahashi et al. 2007). Based on the accumulated 2-D/3-D HPLC data for more than 500 different structures of N-glycans, we developed a web application, GALAXY (Glycoanalysis by the three axes of MS and chromatography), for effective utilization of the multi-dimensional HPLC map (Takahashi and Kato 2003).

Keywords

Sugar Chain High Performance Liquid Chroma HPLC Data Elution Position Linear Multiple Regression Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Kamiya Y, Yamaguchi Y, Takahashi N, Arata Y, Kasai K, Ihara Y, Matsuo I, Ito Y, Yamamoto K, Kato K (2005) Sugar-binding properties of VIP36, an intracellular animal lectin operating as a cargo receptor. J Biol Chem 280:37178–37182PubMedCrossRefGoogle Scholar
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  3. Takahashi N, Yagi H, Kato K (2007) The two/three dimensional HPLC mapping method for the identification of N-glycan structures. In: Kamerling H (ed) Comprehensive glycoscience. Elsevier, AmsterdamGoogle Scholar
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Copyright information

© Springer 2008

Authors and Affiliations

  • Koichi Kato
    • 1
  • Noriko Takahashi
    • 1
  1. 1.Graduate School of Pharmaceutical SciencesNagoya City UniversityNagoyaJapan

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