GALAXY Database and Pyridylaminated Oligosaccharide Library
A wide array of standard oligosaccharides is a valuable glycomics tool for systematic and detailed analyses of structures and functions of sugar chains. The multi-dimensional HPLC mapping method developed by Takahashi and co-workers enables us to isolate N-linked oligosaccharides as pyridylaminated (PA) derivatives, discriminating isomeric structures. Therefore it becomes extremely useful not only for identification and profiling of N-glycans but also for purification and library formation of PA-oligosaccharides (Tomiya et al. 1998; Takahashi et al. 2007). Based on the accumulated 2-D/3-D HPLC data for more than 500 different structures of N-glycans, we developed a web application, GALAXY (Glycoanalysis by the three axes of MS and chromatography), for effective utilization of the multi-dimensional HPLC map (Takahashi and Kato 2003).
KeywordsSugar Chain High Performance Liquid Chroma HPLC Data Elution Position Linear Multiple Regression Analysis
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- Takahashi N, Kato K (2003) GALAXY (Glycoanalysis by the three axes of MS and chromatography): a web application that assists structural analyses of N-glycans. Trends Glycosci Glycotech 15:235–251Google Scholar
- Takahashi N, Yagi H, Kato K (2007) The two/three dimensional HPLC mapping method for the identification of N-glycan structures. In: Kamerling H (ed) Comprehensive glycoscience. Elsevier, AmsterdamGoogle Scholar