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FUT8 Assay

  • Hideyuki Ihara
  • Hiroaki Korekane
  • Akio Matsumoto
  • Naoyuki Taniguchi

Abstract

Mammalian alpha1,6-fucosyltransferase (FUT 8) catalyses the transfer of a fucose residue from a donor substrate guanosine 5′-diphosphate-beta-l-fucose to the reducing terminal N-acetylglucosamine (GlcNAc) of the core structure of an asparagine-linked oligosaccharide. The enzymatic product is called as core-fucosylated N-glycans, which are ubiquitously distributed in many mammalian glycoproteins. Serum levels of fucosylated alpha fetoprotein are very low under normal conditions, but it increases dramatically in malignant diseases and therefore it is one of the best biomarkers for primary hepatomas (see reviews by Miyoshi et al.). Core fucosylation plays a pivotal role in ADCC activity in antibody therapy (see reviews by Sato et al.). KO mice of Fut 8 exhibit severe growth retardation and develop lung emphysema. Crystal structure of this enzyme was reported very recently by our group.

Keywords

Primary Hepatoma Amic Acid High Performance Liquid Chromato Fucose Residue ADCC Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Ihara H, Ikeda Y, Taniguchi N (2006) Reaction mechanism and substrate specificity for nucleotide sugar of mammalian α1,6-fucosyltransferase: a large-scale preparation and characterization of recombinant human FUT8. Glycobiology 16:333–342PubMedCrossRefGoogle Scholar
  2. Miyoshi E, Noda K, Yamaguchi Y, Inoue S, Ikeda Y, Wang W, Ko JH, Uozumi N, Li W, Taniguchi N (1999) Theα1-6-fucosyltransferase gene and its biological significance. Biochim Biophys Acta 1473:9–20PubMedGoogle Scholar
  3. Satoh M, Iida S, Shitara K (2006) Non-fucosylated therapeutic antibodies as next-generation therapeutic antibodies. Expert Opin Biol Ther 6:1161–1173PubMedCrossRefGoogle Scholar
  4. Uozumi N, Yanagidani S, Miyoshi E, Ihara Y, Sakuma T, Gao CX, Teshima T, Fujii S, Shiba T, Taniguchi N (1996a) Purification and cDNA cloning of porcine brain GDP-L-Fuc:N-acetyl-β-d-glucosaminide α1-6fucosyltransferase. J Biol Chem 271:27810–27817PubMedCrossRefGoogle Scholar
  5. Uozumi N, Teshima T, Yamamoto T, Nishikawa A, Gao YE, Miyoshi E, Gao CX, Noda K, Islam KN, Ihara Y, Fujii S, Shiba T, Taniguchi N (1996b) A fluorescent assay method for GDP-L-Fuc:N-acetyl-β-d-glucosaminide α1–6fucosyltransferase activity, involving high performance liquid chromatography. J Biochem (Tokyo) 120:385–392Google Scholar

Copyright information

© Springer 2008

Authors and Affiliations

  • Hideyuki Ihara
    • 1
  • Hiroaki Korekane
    • 1
  • Akio Matsumoto
    • 1
  • Naoyuki Taniguchi
    • 1
  1. 1.Department of Disease Glycomics, Research Institute for Microbial DiseasesOsaka UniversityOsakaJapan

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