Abstract
We investigated the mechanism and impact of various inflammatory stimuli on dendritic cell (DC)-gingival fibroblast (GF) adhesion. Human immature (im) DCs were generated from monocytes by culturing with interleukin (IL)-4 and granulocyte macrophage-colony stimulating factor (GM-CSF). GFs were outgrown from the human gingival specimen. DCs were co-cultured with GFs with/without pretreatment with various stimuli. Adhered cells were measured by fluorometer. Expression of adhesion molecules was analyzed by flow cytometry. Pretreatment of GFs with tumor necrosis factor (TNF)-α, interferon (IFN)-γ, and Escherichia coli (Ec) lipopolysaccharides (LPS) significantly increased the adhesion to imDCs and enhanced intercellular adhesion molecule (ICAM)-1 expression. A signifi-cantly increased DC-GF adhesion was also observed when imDCs were pretreated with Ec LPS, Porphyromonas gingivalis (Pg) fimbriae, and peptideglycan but not with Pg LPS. Expression of LFA-1 and Mac-1 on DCs was not altered by the pretreatment with these stimuli. However, LFA-1 and Mac-1 blockade of imDC signifi cantly reduced the adhesion to TNF-α-stimulated GFs. These results showed that inflammatory stimuli increased the imDC-GF adhesion via lymphocyte function- associated antigen (LFA)-1/Mac-1-ICAM-1 ligation. Adhesion of DC to GFs may be important not only for the localization of DCs in the inflammatory periodontal lesion, but also for the modulation of immune responses.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Godiska R, Chantry D, Raport CJ, et al (1997) Human macrophage-derived chemokine (MDC), a novel chemoattractant for monocytes, monocyte-derived dendritic cells, and natural killer cells. J Exp Med 185:1595–1604
Hart DNJ (1997) Dendritic cells: unique leukocyte populations which control the primary immune response. Blood 90:3245–3287
Jotwani R, Palucka AK, Al-Quotub M, et al (2001) Mature dendritic cells infiltrate the T cell-rich region of oral mucosa in chronic periodontitis: in situ, in vivo, and in vitro studies. J Immunol 167:4693–4700
Takahashi K, Takigawa M, Takashiba S, et al (1994) Role of cytokine in the induction of adhesion molecules on cultured human gingival fibroblasts. J Periodontol 65:230–235
Joe BH, Borke JL, Keskintepe M, et al (2001) Interleukin-1β regulation of adhesion molecules on human gingival and periodontal ligament fibroblasts. J Periodontol 72:865–870
Murakami S, Saho T, Shimabukuro Y (1993) Very late antigen integrins are involved in the adhesive interaction of lymphoid cells to human gingival fibroblasts. Immunology 79:425–433
Hundhausen C, Misztela D, Berkhout TA, et al (2003) The disintegrin-like metalloprotease ADAM10 is involved in constitutive cleavage of CX3CL1 (fractalkine) and regulates CX3CL1-mediated cell-cell adhesion. Blood 102:1186–1195
Marlin SD, Springer TA (1987) Purified intercellular adhesion molecule-1 (ICAM-1) is a ligand for lymphocyte function-associated antigen 1 (LFA-1). Cell 51:813–819
Makgoba MW, Sanders ME, Ginther Luce GE, et al (1988) ICAM-1 a ligand for LFA-1-dependent adhesion of B, T and myeloid cells. Nature 331:86–88
Smith CW, Marlin SD, Rothlein R, et al (1989) Cooperative interactions of LFA-1 and Mac-1 with intercellular adhesion molecule-1 in facilitating adherence and transendothelial migration of human neutrophils in vitro. J Clin Invest 83:2008–2017
Tonnesen MG (1989) Neutrophil-endothelial cell interactions: mechanisms of neutrophil adherence to vascular endothelium. J Invest Dermatol 2:53s–58s
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2007 Springer
About this chapter
Cite this chapter
Minamibuchi, M., Nemoto, E., Kanaya, S., Ogawa, T., Simauchi, H. (2007). Inflammatory stimuli regulate the binding of gingival fibroblasts to dendritic cells via integrin β2. In: Watanabe, M., Okuno, O., Sasaki, K., Takahashi, N., Suzuki, O., Takada, H. (eds) Interface Oral Health Science 2007. Springer, Tokyo. https://doi.org/10.1007/978-4-431-76690-2_37
Download citation
DOI: https://doi.org/10.1007/978-4-431-76690-2_37
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-76689-6
Online ISBN: 978-4-431-76690-2
eBook Packages: MedicineMedicine (R0)