Abstract
Isoforms of amelogenin, a highly conserved protein in mammalians that constitutes 90% of the enamel organic matrix, are mainly produced by ameloblasts. One of the amelogenin splicing variants, leucine rich amelogenin peptide (LRAP) has been reported to promote maturation of osteoblasts and periodontal ligament (PDL) cell proliferation. However, it is still unclear whether the amelogenin splicing variants are involved in chondrogenesis. The aim of the present study was to characterize the function of LRAP in chondrogenesis. Micromass culture of embryonic limb bud mesenchymal cells was performed as in vitro model for chondrogenesis. The recombinant LRAP was added to micromass culture system to analyze the effect on proliferation of mesenchymal cells and chondrogenic differentiation. LRAP increased the number of 5-bromo-2-deoxyuridine (BrdU) positive cells and nodules stained with alcian blue. In addition, LRAP increased mRNA expression of Sox9 and type II collagen. These findings suggest that the amelogenin splicing variant, LRAP, promotes chondrogenesis.
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References
Hatakeyama J, Sreenath T, Hatakeyama Y, et al (2003) The receptor activator of nuclear factor-kappa B ligand-mediated osteoclastogenic pathway is elevated in amelogenin-null mice. J Biol Chem. 278:35743–35748
Hatakeyama J, Philp D, Hatakeyama Y, et al (2006) Amelogenin-mediated regulation of osteoclastogenesis, and periodontal cell proliferation and migration. J Dent Res 85:144–149
Viswanathan HL, Berry JE, Foster B, et al (2003) Amelogenin: a potential regulator of cementum-associated genes. J Periodontol 74:1423–1431
Kim NH, Tominaga K, Tanaka A (2005) Analysis of eosinophilic round bodies formed after injection of enamel matrix derivative into the backs of rats. J Periodontol 76:1934–1941
Hatakeyama Y, Tuan RS, Shum L (2004) Distinct functions of BMP4 and GDF5 in the regulation of chondrogenesis. J Cell Biochem 91:1204–1217
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Hatakeyama, J., Hatakeyama, Y., Haruyama, N., Takahashi, I., Kulkarni, A.B., Sasano, Y. (2007). Amelogenin splicing variant promotes chondrogenesis. In: Watanabe, M., Okuno, O., Sasaki, K., Takahashi, N., Suzuki, O., Takada, H. (eds) Interface Oral Health Science 2007. Springer, Tokyo. https://doi.org/10.1007/978-4-431-76690-2_25
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DOI: https://doi.org/10.1007/978-4-431-76690-2_25
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-76689-6
Online ISBN: 978-4-431-76690-2
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