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Gene Delivery to Chondrocytes Using Adenovirus Vector

  • Conference paper
Book cover Advances in Osteoarthritis

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Summary

The objective of this study was to investigate the effects of adenovirus vector (Ax-)mediated gene transduction of E. coli β-galactosidase (LacZ) and transforming growth factor-β1 (TGF-β1) into a human chondrocyte-like cell line (HCS-2/8). The expression of transduced genes and their expression periods were examined by 5-bromo-4-chloroindolyl-β-D-galactoside (X-gal) staining, Northern blotting, ELISA, and Western blotting. To assess the influence of TGF-β1 gene transduction, the expression of mRNAs of type II collagen, proteoglycan core protein, matrix metalloproteinase-3 (MMP-3), and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) were examined by Northern blotting. Staining with X-gal indicated that the genes were transduced into 99% of the cells. Expression of the transduced genes in the cells was continued for at least 21 days. Transduction of the TGF-β1 gene enhanced mRNA expressions of type II collagen and proteoglycan core protein, but suppressed MMP-3 mRNA expression in the cells. These results indicate Ax is useful in chondrocyte gene therapy, and it could be an efficient mediator of TGF-β1 gene transduction.

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Author information

Authors and Affiliations

  1. Department of Orthopaedic Surgery, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602, Japan

    Toshikazu Kubo, Yuji Arai & Yasusuke Hirasawa

  2. Department of Microbiology, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602, Japan

    Kappei Kobayashi & Jiro Imanishi

  3. Department of Biochemistry and Molecular Dentistry, Okayama University Dental School, 2-5-1 Shikata-cho, Okayama, 700-0914, Japan

    Masaharu Takigawa

Authors
  1. Toshikazu Kubo
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  2. Yuji Arai
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  3. Kappei Kobayashi
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  4. Jiro Imanishi
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  5. Masaharu Takigawa
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  6. Yasusuke Hirasawa
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Editor information

Editors and Affiliations

  1. Kinki University School of Medicine, 377-2 Ohno-Higashi, Osaka-Sayama, 589-8511, Osaka, Japan

    Seisuke Tanaka M.D. (Dean) (Dean)

  2. Department of Orthopedic Surgery, Kinki University School of Medicine, 377-2 Ohno-Higashi, Osaka-Sayama, 589-8511, Osaka, Japan

    Chiaki Hamanishi M.D. (Professor) (Professor)

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© 1999 Springer-Verlag Tokyo

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Kubo, T., Arai, Y., Kobayashi, K., Imanishi, J., Takigawa, M., Hirasawa, Y. (1999). Gene Delivery to Chondrocytes Using Adenovirus Vector. In: Tanaka, S., Hamanishi, C. (eds) Advances in Osteoarthritis. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68497-8_8

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  • DOI: https://doi.org/10.1007/978-4-431-68497-8_8

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-68499-2

  • Online ISBN: 978-4-431-68497-8

  • eBook Packages: Springer Book Archive

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