Summary
Expression of the e-Fos protein was examined by immunohis-tochemistry in rat knee joints with surgically induced osteoarthritis. Cells stained for the c-Fos protein were not observed in the control knees. However, in early osteoarthritic joints, chondrocytes that expressed c-Fos protein were observed in all the articular cartilage layers on day 3 and in the residual superficial layers 2 weeks after surgery. Safranin-O staining of the matrix decreased in the superficial layers in which c-Fos-positive chondrocytes were identified. In those knee joints in which potent protein kinase C (PKC) agonist 12-o-tetradecanoylphorbol-13-acetate (TPA) was injected intraarticularly, significantly fewer histological osteoarthritic changes developed 2 and 4 weeks after surgery, and the numbers of chondrocytes that expressed c-Fos protein generally decreased and were observed mainly in the superficial layers even on day 3 and were not observed in any of the articular cartilage layers 2 and 4 weeks after surgery. Safranin-O staining was normally observed even 2 weeks after surgery. These chondroprotective effects of exogenous TPA appeared to be related to the inhibition of c-Fos protein synthesis in chondrocytes.
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© 1999 Springer-Verlag Tokyo
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Hamanishi, C., Miyazaki, H., Satsuma, H., Tanaka, S. (1999). Protein Kinase C-Activator Inhibits Progression of Osteoarthritis by Inhibiting c-Fos Protein Expression in Articular Chondrocytes. In: Tanaka, S., Hamanishi, C. (eds) Advances in Osteoarthritis. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68497-8_14
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DOI: https://doi.org/10.1007/978-4-431-68497-8_14
Publisher Name: Springer, Tokyo
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